Shown is the result of Messelson & Stahl experiment for an ancient species discovered in thethawing permafrost: The Messelson and Stahl experiments labelled the parental DNA of thespecies with N15- DNA. Then the organism was transferred to a culture medium containingN14-NH4(S04) as the only source of nitrogen for the newly synthesized DNA.N14N14N14N15N15N15N15FoF1F2What are the chances of the species evolving to new species or become extinct? Elaborate.F3

Meselson and Stahl experiment confirmed the results that the DNA replication is semi-conservative in…

Answered: Shown is the result of Messelson &…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Most yeast grow suspended in culture, but some yeast grow as a film on the top of a liquid culture. These are called flor yeast. The following Southern blot is looking at the FLO11 gene that is thought to be involved in whether yeast are flor or not. DNA was isolated from four different yeast strains. Strains 1 and 3 are normal while strains 2 and 4 are flor (Fidalgo, 2006). Based on the gene map and Southern results below, what aspect of FLO11 determines whether a strain will be flor or not?
In E. coli, Base excision repair (BER) handles non-bulky lesions (e.g., deamination, oxidation) and nucleotide excision repair (NER) handles bulky lesions (e.g., Py dimers). Is this statement true or false? O True False
Which of the strands of DNA could act as a PCR primer for the DNA sequence shown below? 5’-CCCTGGGCTCTGTAAATGTTTCTAAGTG-3’ 3’-GGGACCCGAGACATTTACAAAGATTCAC-5’ A. 3′-ACTGTTAGA-5′ B. 3′-AAATTTGGC-5′ C. 3′-ATGCTTTGA-5′ D. 5′-GGGACCCGA-3′ E. 5′-CCCTGGGCT-3′
An archaeon (Sulfolobus acidocaldarius) found in acidic hot springs contains a topoisomerase that catalyzes the ATP-driven introduction of positive supercoils into DNA. How might this enzyme be advantageous to this unusual organism?
We isolate DNA from a bacteria that is wild type for the genes arg leu and his shear it in a blender mix it into a culture of bacteria that is mutant for the genes arg leu and his . We then plate on various media. These are the results: Media with arginine 20 colonies /ml Media with histidine 300 colonies /ml Media with leucine 500 colonies /ml Media with all three 10000 colonies/ml 21. What is this experiment called? A. transpiration B. conjugation C. transformation D. transduction
Some bacteria may have higher mutation rates than others following exposure to UV light. Discuss a reason why this might be the case. What experiments could you do to determine whether this is a likely possibility?
You purified the genome of the coliphage X174. After analysis, you find that its composition is 25 percent A, 33 percent T, 24 percent G, and 18 percent C. Based on this finding, which of the following prediction would you make for the coliphage $X174? The genome of coliphage X174 is double-stranded DNA. The genome of coliphage X174 is single-stranded DNA. The genome of coliphage X174 is single-stranded RNA. The genome of coliphage $X174 is double-stranded RNA. Previous Page Next Page Page 9 of 30
Will you please help me in understanding these? How many fragments will be formed after digestion in sample C, if the plasmid is circular and why? What are the critical steps in transformation? How was RAD52 expression observed? Provide current applications of DNA Technology
In a process of production of a recombinant protein by E. coli cells, it was observed accumulation of acetate in the culture medium. In this situation, it can be said that: (a) certainly the process in question was being conducted in anaerobiosis (B).Acetate accumulation is advantageous for the process as the acetate formation reaction generates 1 molecule of ATP (c)Knowing that decreasing the temperature of the process causes a reduction in the rate of glycolysis, this could be a strategy to reduce the accumulation of acetate (d).the acetate formed can be re-assimilated by the cell if the glyoxylate pathway is activated at some point in the culture
Consider the ends of the DNA fragments shown below. They have been produced by digestion of a single sequence of DNA using a number of restriction endonucleases. 1. 5'A 3' 3'TTCGA5' 2. 5'G 3' 3'CAGCT5' 3. 5'AATTC3' 3' G5 4. 5'TCGAC3' 3' G5' 5. 5'GGG 3' 3'CCC 5' Which of these ends are capable of annealing and being joined by DNA ligase?
PCR is a molecular biology technique where template DNA is amplified using a primer and oligonucleotides. The reaction is catalyzed by a thermostable DNA polymerase and in a particular reaction, the template strands are denatured at 95˚C. For strand hybridization, the melting temperature is 55˚C. What do you predict about the average duration of H bonds at the high temperature in comparison to the low temperature?

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