Based from Fig. 1B, which of the following lanes contain a good quality genomic DNA? Bad quality genomic DNA? Why or Why not? Answer in maximum of 5 sentences only. You can prove your answer by describing the banding pattern of each lanes and how to troubleshoot the DNA extraction procedure if possible.
Based from Fig. 1B, which of the following lanes contain a good quality genomic DNA? Bad quality genomic DNA? Why or Why not? Answer in maximum of 5 sentences only. You can prove your answer by describing the banding pattern of each lanes and how to troubleshoot the DNA extraction procedure if possible.
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Based from Fig. 1B, which of the following lanes contain a good quality genomic DNA? Bad quality genomic DNA? Why or Why not? Answer in maximum of 5 sentences only. You can prove your answer by describing the banding pattern of each lanes and how to troubleshoot the DNA extraction procedure if possible.
![1
2345M
12345678M
1500bp
1000bp
1500bp
1000bp
500bp
500bp
100bp
100bp
A
B
Figure 1. AGE result of conventional PCR (A) and the extracted genomic DNA (B) along 1.5kb ladder.](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fc69161e8-d9b9-443e-8953-e5f4d638fdc4%2F206c947e-5332-42d1-aad3-2e7ad47f04ca%2F06x89h8_processed.jpeg&w=3840&q=75)
Transcribed Image Text:1
2345M
12345678M
1500bp
1000bp
1500bp
1000bp
500bp
500bp
100bp
100bp
A
B
Figure 1. AGE result of conventional PCR (A) and the extracted genomic DNA (B) along 1.5kb ladder.
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What about lanes 3 and 4 in figure B? Also, what other factors causes smearing except from RNA and protein contamination?
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