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Question 19. You need to prepare sequencing libraries of two different DNA fragment sizes. One library should contain DNA fragments of approximately 800 bp and the other 5000 bp. You are going to fragment your DNA using a nebulizer. Indicate the correct statement(s) below: a. To obtain a library of 800 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of the 5000 bp fragment library. b. To obtain a library of 5000 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of an 800 bp fragment library. c. The gas pressure used during nebulization does not influence the size of the DNA fragments. d. The size of the DNA fragments obtained in the process of nebulization depends on the GC content of the DNA. e. The size of sheared DNA molecules is inversely proportional to the size of the droplets formed during nebulization.

Question 19. You need to prepare sequencing libraries of two different DNA fragment sizes. One library should contain DNA fragments of approximately 800 bp and the other 5000 bp. You are going to fragment your DNA using a nebulizer. Indicate the correct statement(s) below: a. To obtain a library of 800 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of the 5000 bp fragment library. b. To obtain a library of 5000 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of an 800 bp fragment library. c. The gas pressure used during nebulization does not influence the size of the DNA fragments. d. The size of the DNA fragments obtained in the process of nebulization depends on the GC content of the DNA. e. The size of sheared DNA molecules is inversely proportional to the size of the droplets formed during nebulization.

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Question 19. You need to prepare sequencing libraries of two different DNA fragment sizes. One library should contain DNA fragments of approximately 800 bp and the other 5000 bp. You are going to fragment your DNA using a nebulizer. Indicate the correct statement(s) below: a. To obtain a library of 800 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of the 5000 bp fragment library. b. To obtain a library of 5000 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of an 800 bp fragment library. c. The gas pressure used during nebulization does not influence the size of the DNA fragments. d. The size of the DNA fragments obtained in the process of nebulization depends on the GC content of the DNA. e. The size of sheared DNA molecules is inversely proportional to the size of the droplets formed during nebulization.
Transcribed Image Text:Question 19. You need to prepare sequencing libraries of two different DNA fragment sizes. One library should contain DNA fragments of approximately 800 bp and the other 5000 bp. You are going to fragment your DNA using a nebulizer. Indicate the correct statement(s) below: a. To obtain a library of 800 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of the 5000 bp fragment library. b. To obtain a library of 5000 bp fragments, you need to apply lower gas pressure during the nebulization process than for preparation of an 800 bp fragment library. c. The gas pressure used during nebulization does not influence the size of the DNA fragments. d. The size of the DNA fragments obtained in the process of nebulization depends on the GC content of the DNA. e. The size of sheared DNA molecules is inversely proportional to the size of the droplets formed during nebulization.
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