Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?
Background: The following results were obtained from a broth dilution test for microbial susceptibility. What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
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Background: The following results were obtained from a broth dilution test for microbial susceptibility.
What is the minimum bactericidal concentration of this antibiotic and explain what you based your answer on?
Transcribed Image Text:### Antibiotic Concentration and Bacterial Growth
The table presents data on the effect of different antibiotic concentrations on bacterial growth and their subsequent growth in subculture. The antibiotic concentrations are measured in micrograms (μg).
| Antibiotic Concentration | Growth | Growth in Subculture |
|--------------------------|--------|----------------------|
| 200 μg | - | - |
| 100 μg | - | - |
| 50 μg | - | + |
| 25 μg | + | + |
**Explanation:**
- **200 μg & 100 μg**: At these high concentrations, there is no bacterial growth initially, nor in the subculture, indicating effective bactericidal activity.
- **50 μg**: No initial growth is observed, but growth occurs in the subculture, suggesting some surviving bacteria may not grow initially but can recover once transferred.
- **25 μg**: Growth is noted at this lower concentration both initially and in subculture, indicating insufficient antibiotic strength to inhibit or kill the bacteria effectively.
This table can be used for understanding the minimum inhibitory concentration (MIC) and the bacteriostatic versus bactericidal nature of the antibiotic at varying concentrations.
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