Answer the following questions, showing work in the form of calculations. a) At what substrate concentration would an enzyme with a Vmax of 102.5 M/min and a Km of 0.0025 M operate at one-quarter of its maximum rate? b) Determine the fraction of Vmax that would be obtained at the following substrate concentrations [S]: 0.5Km, 2Km, and 10Km

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Q: [S] mM 20 50 100 200 300 500 700 Initial Velocity (mM/min) 1.012 1.950 2.823 3.637 4.024 4.398 4.580

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