After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?
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After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?
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- IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?Which of the following statements is/are FALSE about Bradford assay? 1. Upon addition of the reagent, the color of the protein solution becomes purple. II. The absorbance of the resulting solution is measured at a wavelength of 540-550 nm. Both I and II O II only Neither I nor II O I onlyDuring incubation, prepare dilutions of the standard antiserum which constitutes the standard curve for the assay (concentration in ng/mL). Add 500 µL of PBS-milk to the supplied microtube containing 500 µL of standard antiserum to obtain Standard 1 [500 ng/mL]. Identify seven microtubes for standards 2 to 7 and place 500 µL of PBS-milk in each. Calculate how much of antiserum is used in each standard ?
- why does a GC rich (70-80%) and GC poor (30-40%) give a low read coverage?A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial activity. Live bacterial cells are detected using “Live clear", a dye which is initially coloured blue, but turns clear in the presence of live bacterial cells. The control wells are shown below. Penicillin kills bacteria; glycerol does not affect them. Which of the control wells will appear blue at the end of the assay? "Live clear" glycerol penicillin - - + (A1 A2)(A3)A4) bacterial cells B1 (B2 (B3)B4 А4, В1, B2, ВЗ, B4 A2, A4, B2, B3, B4 А4, B2, ВЗ, В4 A1, АЗ, В1, В2, вЗ, В4 + + |Calculation for a neutral red assay please include steps since it is essential for the report and it is marked.
- For tha analysis of a specific drug present in different pharmaceutical preparations, explain when is more appropriate to use absorbtion based assay and when is more appropriate to use a fluorescence based assay.What are the different categories of cell viability assays? Describe one of them briefly. Define the role (aim of use) of Trypan blue dye in cell culture studies.If you mix two unknown samples and repeat the Lowry assay, is the absorbance equivalent to the sum of the two individual unknown samples that is used.
- is bradford assay using dye Coomassie Brilliant Blue G-250 a different method from using a spectrophotometer ? if so how is it different to determine protein concentration?What is the reason for using sterilized filter paper in CHORIOALLANTOIC MEMBRANE (CAM) Assay?A plaque assay is performed beginning with 1 mL of a solution containing bacteriophages. This solution is serially diluted three times by combining 0.1 mL of each sequential dilution with 9.9 mL of liquid medium. Then 0.1 mL of the final dilution is plated in the plaque assay and yields 17 plaques. What is the initial density of bacteriophages in the original 1 mL?