After incubation, the plates appeared as below: Water EvaGreen Ethidium Bromide SYBR Green 1
Molecular Techniques
Molecular techniques are methods employed in molecular biology, genetics, biochemistry, and biophysics to manipulate and analyze nucleic acids (deoxyribonucleic acid (DNA) and ribonucleic acid (RNA)), protein, and lipids. Techniques in molecular biology are employed to investigate the molecular basis for biological activity. These techniques are used to analyze cellular properties, structures, and chemical reactions, with a focus on how certain molecules regulate cellular reactions and growth.
DNA Fingerprinting and Gel Electrophoresis
The genetic makeup of living organisms is shown by a technique known as DNA fingerprinting. The difference is the satellite region of DNA is shown by this process. Alex Jeffreys has invented the process of DNA fingerprinting in 1985. Any biological samples such as blood, hair, saliva, semen can be used for DNA fingerprinting. DNA fingerprinting is also known as DNA profiling or molecular fingerprinting.
Molecular Markers
A known DNA sequence or gene sequence is present on a chromosome, and it is associated with a specific trait or character. It is mainly used as a genetic marker of the molecular marker. The first genetic map was done in a fruit fly, using genes as the first marker. In two categories, molecular markers are classified, classical marker and a DNA marker. A molecular marker is also known as a genetic marker.
DNA Sequencing
The most important feature of DNA (deoxyribonucleic acid) molecules are nucleotide sequences and the identification of genes and their activities. This the reason why scientists have been working to determine the sequences of pieces of DNA covered under the genomic field. The primary objective of the Human Genome Project was to determine the nucleotide sequence of the entire human nuclear genome. DNA sequencing selectively eliminates the introns leading to only exome sequencing that allows proteins coding.
In order to determine which dye (Ethidium bromide (10 µg/ml), EvaGreen (10 µg/ml) or SYBR Green (10 µg/ml) is the safest to use in a laboratory for DNA binding, first sterilise the spreader and forceps with 95% ethanol, then place 2-3 drops of bacterial culture on each plate and spread well with a sterile spreader and using the sterile forceps, place a sterile filter disk on the center of each plate. Next, Add a drop of sterile water to the filter paper on the control plate, a drop of ethidium bromide to another plate, a drop of EvaGreen to another plate, and a drop of SYBR green to another plate. The sterile water is used as a negative control. And then incubate the plates at 37°C for 48 hours.
The results obtained are attached as a picture, after looking at the results obtained in the picture;
use the Ames Assay to determine which of the provided dyes are safest?
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