Which statement is true regarding pros vs. cons of SYBR green vs. probe-based qPCR? O A. SYBR green QPCR assays are generally easier/cheaper to use and design while fluorescent probe assays are generally more complicated to design O B. Fluorescent probes allow for multiplexing (measuring more than one target in the same QPCR well) while SYBR green does not allow for that. OC. SYBR green-based qPCR assays are measured using Ct values, while fluorescent probe-based assays are not Fluorescent probes are highly specific and do not tolerate mutations at the binding site while SYBR green-based qPCR is more likely to include nor OD. amplicons O E. Fluorescent-probe based assays rely on fluorescence while SYBR green-based qPCR does not O F. All of the above O G.A, B, and D
Which statement is true regarding pros vs. cons of SYBR green vs. probe-based qPCR? O A. SYBR green QPCR assays are generally easier/cheaper to use and design while fluorescent probe assays are generally more complicated to design O B. Fluorescent probes allow for multiplexing (measuring more than one target in the same QPCR well) while SYBR green does not allow for that. OC. SYBR green-based qPCR assays are measured using Ct values, while fluorescent probe-based assays are not Fluorescent probes are highly specific and do not tolerate mutations at the binding site while SYBR green-based qPCR is more likely to include nor OD. amplicons O E. Fluorescent-probe based assays rely on fluorescence while SYBR green-based qPCR does not O F. All of the above O G.A, B, and D
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:QUESTION 17
Which statement is true regarding pros vs. cons of SYBR green vs. probe-based qPCR?
A. SYBR green qPCR assays are generally easier/cheaper to use and design while fluorescent probe assays are generally more complicated to design
O B. Fluorescent probes allow for multiplexing (measuring more than one target in the same qPCR well) while SYBR green does not allow for that.
O C. SYBR green-based qPCR assays are measured using Ct values, while fluorescent probe-based assays are not
Fluorescent probes are highly specific and do not tolerate mutations at the binding site while SYBR green-based qPCR is more likely to include non-specific
OD.
amplicons
E. Fluorescent-probe based assays rely on fluorescence while SYBR green-based qPCR does not
O F. All of the above
O G.A, B, and D
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