Answered: How is the Bicinchoninic acid assay… | bartleby

Basic Clinical Laboratory Techniques 6E

Basic Clinical Laboratory Techniques 6E

6th Edition

ISBN:9781133893943

Author:ESTRIDGE

Publisher:ESTRIDGE

Chapter2: Basic Hematology

Section2.3: Microhematocrit

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How is the Bicinchoninic acid assay used in protein quantification?

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Which of the following are true regarding assay attributes? List all that are true. a) Accuracy represents the closeness of the assay result to the “true value”. b) Robustness represents the ability of a method to distinguish between the analyte and similar components. c) Precision is determined by replicate analysis of a reference standard or well-characterized material. d) A robust assay would have a very narrow range of acceptable assay conditions. For those that were not true in question 1, correct the statement(s).
At higher amounts of protein, the Bradford assay is not linear. Consider the plot to the right: what is the maximum amount of protein a sample could contain and still fall within a standard curve? Briefly explain your reasoning.
For what purposes are protein microarray chips used?
What does MEGA stand for and what is this assay used to determine? How to do perform the assay?
There are three names that often get used when discussing the Lowry assay. Those names are: Lowry, Folin-Ciocalteu, and Biuret. Are those names interchangeable when discussing the assay or do those names describe the same thing(s)? Significant differences? What is the relationship between those names?
Describe the p-nitrophenol assay and how a standard curve can be used to calculate the concentration of an unknown .
Why is it not necessary to dilute your protein samples with buffer in a bradford protein assay experiment?
Describe two different types of protein microarrays, and discuss their uses.
Describe the two general types of protein microarrays. What aretheir possible applications?
Why is it necessary to examine gene-expression profiles, in additionto genome sequences, for effective precision medicine?
If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be done?
Why is the bradford assay method using BSA sensitive ? and how does it compare to the amino acid analysis method

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