How do shotgun and directed sequencing differ? Why is coverage an important issue?

Q: is possible to buy a CRISPR kit that you can do in your own home. Is this a good idea? After…

A: In 2013, the Zhang lab successfully published the first method to engineer CRISPR to edit the genome…

Q: Describe the shotgun method for sequencing an entire genome.

A: Genome or genetic material of a cell is the DNA content present in the nucleus. DNA or…

Q: Which is a limitation of using massively parallel sequencing ?

A: It is a term used to describe the method of high-throughput DNA sequencing to determine the entire…

Q: What are STRs, and why are they useful for DNA profiling?

A: Introduction  In the genome there are usually two type of nucleic acid sequence present. One is Non…

Q: What is southern blot? Understand PCR reaction in detail. What polymerase enzyme is used in PCR…

A: Both southern-blotting and PCR are types of recombinant DNA technology. Recombinant DNA technology…

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: DNA is the genetic substance of all creatures. It is a double-helical structure made up of two…

Q: What are the emerging techniques and challenges in utilizing multi-omics data for comprehensive…

A: The rapid advancements in sequencing technologies have allowed us to generate a wealth of data on…

Q: Differentiate between Sanger sequencing and next generation sequencing?

A: The process of determining the nucleic acid sequence is known as DNA sequencing.

Q: Describe the automated dideoxy chain termination method of DNA sequencing.

A: The chain termination DNA sequencing method is the Sangers sequencing in which the DNA fragment is…

Q: Why are entire genomes not used for DNA profiling?

A: DNA profiling is the process of characterizing a sequence of DNA obtained from a person. It is also…

Q: Is whole-genome sequencing for an individual beneficial? Why or why not?

A: The process of whole-genome sequencing (WGS) allows for a thorough examination of the complete…

Q: What is hierarchical sequencing?

A: Basically there are two approaches for the sequencing of large complex genome. They are hierarchical…

Q: What are the possible bioethical issues that gene editing tools may encounter?

A: Gene editing tools are used to edit an organisms genetic code , i.e.. with these tools ,the genetic…

Q: What are gene microarrays? How are microarrays used?

A: The process of forming a single double-stranded nucleic acid molecule by combining two…

Q: What provides a convenient bridge between the low resolution of a karyotype and the ultra-high…

A: Introduction  Cytology refers to the study of cell such as cell morphology, physiology etc. As we…

Q: Discuss the concept of the use of DNA in genome identification.

A: DNA (Deoxyribo nucleic acid) is a double stranded genetic element in all prokaryotic and eukaryotic…

Q: What is domain? And Why is it complicated to classify and study domains purely using sequence…

A: All the prokaryotic organisms belong to Archea or Bacteria while eukaryotic organisms belong to…

Q: How to create DNA-sequence alignment ?

A: DNA-Sequence alignment can be defined as the way in which sequences of DNA samples are arranged for…

Q: Explain the difference between sequencing by chain termination and sequencing by synthesis. Which…

A: Chain termination process employs dideoxynucleoside triphosphates (ddNTPs) lacking OH (hydroxyl)…

Q: Why a multiple sequence alignment is needed for researchers? What inferences can be derived from…

A: When three or more biological sequences are aligned and studied together, it is referred to as…

Q: What is the purpose of shotgun sequencing?

A: Deoxyribonucleic acid (DNA) sequencing is the process of determining the nucleic acid sequence based…

Q: Define the Single-cell sequencing (SCS) ?

A: Working of Single-cell deoxyribonucleic acid genome sequencing involves analytic one cell,…

Q: What is Human Genome Project? What are its goals and benefits? What are the ethical, legal, and…

A: The genome is made up of genes and genes are made up of DNA. DNA is present inside the chromosomes…

Q: What does whole exome sequencing test for?

A: Exomes are part of the genome, which contains only the coding portions of the genes that is the…

Q: How the Sequencing Technologies Have Progressed Rapidly ? Explain about this ?

A: Early efforts at sequencing genes ere troublesome, when Gilbert and Maxam reported the sequence of…

Q: process of overlap assembly in whole genome sequencing

A: Genome sequencing Genome sequencing or gene sequencing is a way of arranging nucleotides the n the…

Q: What does whole exome sequencing reveal?

A: Molecular biology techniques are the techniques that utilize the genetic material of an organism…

Q: Explain the sequencing-by synthesis (SBS) approach ?

A: One of the well-known states that are known as fundamental topics in Arithmetic is sequence and…

Q: analyzing sequencing data, what parameters should be considered?

A: Genomic research has been transformed by improvements in DNA sequencing technology, which have made…

Q: What is genomic sequencing and what are the 3 primary ways of annotating a gene?

A: A genome sequence:It is the total nucleotides list that include A, C, G, and T for the DNA genomes…

Q: Outline different strategies for identifying gene sequences.

A: Genome is the study of all genes present in the organism. Gene is a unit of heredity that is…

Q: What is the motive behind Genome Project 10K?

A: The advent of low-cost, high throughput sequencing has ushered in a new age of genome science and…

Q: Why is GenBank important and What is GenBank format?

A: GenBank is the Genetic sequence database at NCBI (National center for biotechnology information). It…

Q: How many male/female sheep are needed to clone one? Cancloning be done by using adult cells?

A: One male and one female sheep is required to clone one because we need one male gamete and one…

Q: A strain of mice has been developed that lack the enzyme phosphorylase kinase. Yet, after strenuous…

A: Glycogen is considered as the energy storage for the animal cells because it helps to supply glucose…

Q: How are dideoxynucleotide triphosphates utilized in sequencing?

A: The chain elongating inhibitors of the DNA polymerase which are required for the Sanger method of…

Q: When is low diversity especially problematic in sequencing?

A: In sequencing micro RNA any method which requires use of in house barcodes may results in giving low…

Q: What is maxam and gilbert method of sequencing

A: DNA (deoxyribonucleic acid) sequencing is a procedure to determine the order of nucleotides…

Q: What is the one main advantage of the whole-genome shotgun strategy over the hierarchical approach?

A: Genome sequencing is a vital process that helps in biological and medical analysis.

Q: How new sequencing methodologies are replacing traditional genomic DNA libraries ?

A: The term genomic DNA library is associated with DNA fragments collection comprising an organism's…

Genetic Evolution

Sexual reproduction produces distinct population variations. Heredity determines the traits and qualities to be passed down to the progeny. Over time, this variation within-species is also affected by the process of human evolution. The study of how genetic variation leads to evolutionary changes is known as genetic evolution. If a trait helps an organism to survive and reproduce, it is more likely to be passed down the generations. It is rare that such traits result in a completely distinct set of traits in descendants than the ancestral population; this can result in the emergence of a new species.