How do shotgun and directed sequencing differ? Why is coverage an important issue?

Answered: How do shotgun and directed sequencing…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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How do shotgun and directed sequencing differ? Why is coverage an important issue?

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Step 1: Introduction

Shotgun sequencing and directed sequencing are two different approaches in the field of DNA sequencing.

Shotgun Sequencing:
- Method: Shotgun sequencing involves randomly breaking the DNA into small fragments, sequencing those fragments, and then reassembling the sequences to construct the complete genome.
- Randomness: The key feature is randomness; there's no prior knowledge of the order or location of the sequences being analyzed.
- Use: This method is typically used for sequencing whole genomes, especially in larger and more complex genomes, like those of humans or other organisms.

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