Explain the basic principles of Elispot Assay
Q: Discuss the E-test of antibiotic testing. pls make it clear, concise and organize
A: Antibiotic testing is done to assess the usage of various doses of antibiotics for different…
Q: Among various dye-based assays like Biuret, Lowry, Bicinchronic Acid, and Biuret Protein Assays,…
A: Biuret, lowry, and bicinchoninic acid-based assays are used to quantify the protein concentration in…
Q: Define about immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) assays ?
A: Introduction: A method of dissecting a substance to decide its structure or quality is known as an…
Q: Does TGF-β treatment cause cells to grow more or less in the soft-agar assay? (a) More, (b) Less
A: Anchorage-independent growth, which is a hallmark of carcinogenesis, is the capacity of transformed…
Q: Discuss the possible advantages of quorum sensingproteins as antibiotic targets
A: Quorum sensing enables the bacteria to detect and respond to the cell population density by gene…
Q: Explain the steps of preparing a cell culture stock from a T25 flask.
A: Cell culture is the specialized process used in molecular biology to remove cells from plants and/or…
Q: Describe what CRISPR is and explain the overall process of how it works. Identify and discuss 3-4…
A: CRISPR : It stands for Clustered Regularly Interspaced Short Palindromic Repeats Repetitive DNA…
Q: explain the method of Identifying mutant genes by plasmid librarytransformation
A: Transformation is the horizontal gene transfer by which some bacteria can take up foreign genetic…
Q: Describe the principles behind direct and indirect fluorescent antibodytests.
A: The principle used in fluorescence microscopy is that fluorescence dyes absorb invisible UV light of…
Q: Define about agarose gel electrophoresis ?
A: Electrophoresis is an important tool in biology. It involves separating basic biomolecules: nucleic…
Q: What are the vectors used in rDNA technology? briefly explain each
A: rDNA technology or Recombinant DNA technology is a widely used technology with the advancement of…
Q: TGF-beta treatment cause cells to grow more or less in the soft-agar assay
A: TGF beta The transforming growth factor-beta is a cytokine a protein which is produced by all types…
Q: Explain how in vivo testing differs from in vitro testing.
A: In vitro and In vivo are the methods used in scientific studies for the research. The various…
Q: Describe how a plaque assay is used to detect and estimate the numbers of phage particles.
A: Plaque is a term used in microbiology for a clear zone on a bacterial culture plate that indicates…
Q: Discuss shortly five important things that should be kept in mind when designing a centrifugation…
A: Introduction: The method used for the detachment of an elements of an analyte dependent on contrasts…
Q: A high-throughput assay is being conducted in a 96 well plate to test compounds for anti-bacterial…
A: It is given that “live clear” which is initially a blue-colored dye, turns clear in the presence of…
Q: Explain the steps involved in monoclonal antibody production using hybridoma technology.
A: Monoclonal antibodies are artificial antibodies designed to identify and bind to any antigens…
Q: Compare and contrast ex vivo and in vivo gene therapy as approaches for delivering therapeutic…
A: Viral vectors are used as carriers for normal genes that replace the defective genes within the…
Q: How are molecular probes prepared?
A: Molecular probes are small segments of deoxyribonucleic acid (DNA) or ribonucleic acid (RNA), which…
Q: Discuss some advantages and disadvantages of bacteriophage therapyin treating bacterial infections.
A: Bacteriophage is defined as the type of virus that infects bacteria. It is also called as bacterial…
Q: Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance…
A: Antibiotic resistance The term antibiotic resistance is made up of two words i.e. resistance(ability…
Q: Describe the use of peptide nucleic acid probes in clinical microbiology
A: Peptide Nucleic Acid Probes (PNAPs) are the artificial replicas of the DNA where the deoxyribose…
Q: isolation and purification of bacteriophages from sewage through enrichment centrifugation and the…
A: Isolation of Bacteriophages Centrifuge the Sewage samples at 12000 x g for 30 minutes. It will…
Q: Explain the relative importance of the bioassay during the different phases of research and…
A: Bioassays are used to determine the concentration of a substance by exerting its effect on the…
Q: describe in detail the labeling reaction used to label your probe.
A: DNA probing is the method where the DNA sequence can be modified or tagged using different chemical…
Q: What are the optimal conditions for EcoRV and Aval restriction enzymes?
A: Restriction enzymes are also known as molecular scissors since they are used in cutting specific…
Q: Briefly describe the principle of the MTT assay and how this assay helps you determine the LC50 of…
A: Testing cytotoxicity of a specific chemical or drug is a routine procedure in several biochemistry…
Q: If a concentrated sample is out of the detection range of a chosen dye-based assay, what can be…
A: Dye based assay is a dye binding protein assay based on the binding of protein molecules to…
Q: What is the purpose of Southern's blotting technique? Explain in detail the biochemical principle…
A: The purpose of Southern blotting technique is to identify specific DNA from the mixture of other…
Q: Briefly describe how toe Bradford protein assay works
A: Introduction There are several methods to determine various biomolecules either quantitatively or…
Q: Discuss the main phases (steps) of micropropagation briefly?
A: All organisms are made up of numerous cells. All cells further organize to form organs and organ…
Q: What is high throughput screening in the discovery of drug ?
A: Drug discovery is a highly complex and multidisciplinary process which goal is to identify new…
Q: escribe how the bacteria should have the ability to be recovered back from the infected host for the…
A: Bacteria are microscopic organisms which belong to prokaryote because these are unicellular…
Q: Explain about RNA-induced silencing complex (RISC) ?
A: The RISC is expanded as RNA-induced silencing complex. This is referred to as a multiprotein…
Q: In the plaque assay, exactly what makes up a single plaque?
A: Viruses are non-cellular pathogenic particles. Viruses cannot survive on their own as they lack cell…
Q: . What is the full form of RFLP?
A: Restriction enzymes are the endonucleases that cleave DNA specific locations. random DNA mutations…
Q: STEP 1 YFP Trarsfect into ouilts STEP 2 Epitope-tagged protain (ancoded by eDNA) STEP 3 Lyse calls…
A: Protein is a polymer formed from amino acid joined by peptide bond. It is abundantly found in the…
Q: In ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the…
A: ELISPOT assay is an Enzyme-Linked Immune adsorbent Spot assay that is used for testing cellular…
Q: ecombinant DNA technology has provided a broad range of tools to help physicians in the diagnosis of…
A: Every pathogen has some unique sequence in their DNA that can be targeted by using probes. Thus…
Q: Describe an electrochemical sensor assay method for rapid bacterial detection and identification.…
A: Electrochemical sensors work on the principle of sensing, recognition and production of electrical…
Q: How is a specific probe obtained?
A: Probe is a single-stranded sequence of DNA or RNA used to search for its complementary sequence in a…
Q: Compare and contrast the advantages and disadvantages of production of genetically modified crps.
A: Genetically modified crops (GM crops) are produced by genetic alteration of DNA. This aims to…
Q: Discuss the applications of molecular diagnostic tests
A: Introduction :- Molecular diagnostic tests refers to the diagnosis of a disease at the molecular…
Q: Discuss briefy how a probe is used in molecular diagnostics.
A: A probe is an oligonucleotide stretch of DNA (Deoxyribonucleic Acid) or RNA (Ribonucleic Acid) which…
Q: What is single molecule sequencing in real time (SMRT) ?
A: DNA sequencing is a technique for determining the order of the four nucleotide bases found in DNA:…
Q: Explain the purposes behind replica plating and the Ames test.
A: Replica plating is a microbiological technique in which one or more secondary Petri plates…
Q: How is bacterial transformation used in biotechnology and How can we tell if we have successful…
A: Bacterial transformation may be referred to as a stable genetic change brought about by the uptake…
Explain the basic principles of Elispot Assay
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- With detail, compare and contrast the following 5 real-time assays; Taqman, SYBR Green, Molecular Beacon, FRET, and Scorpion. Please write complete sentences in paragraph form. Identify how the assays are similar but more importantly, what are the distinguishing features of each of the technologies.In ELISPOT Assay, explain the importance of adding stimulus to the 96-well plate during the incubation of Peripheral Blood mononuclear cellsWhat is an ONPG test? WHat is he principle behind this assay.
- After adding stop solution (sulphuric acid) in reaction mixture of HRP assay with TMB, yellow color becomes brighter. What is the mechanism?Discuss how DNA extraction of microbial DNA directly can be done from infected tissue through an optimized protocol for use in nanopore sequencing.Explain: Describe an electrochemical sensor assay method for rapid bacterial detection and identification. What are the principles and mechanisms involved? functionalization of a sensor array with DNA oligonucleotide capture probes for ribosomal RNA (rRNA) species-specific sequences. sandwich hybridization of target rRNA with the capture probe and a horseradish peroxidase linked DNA oligonucleotide detector probe. Explain how bacteria are being detected using an electrochemical sensor
- Briefly describe the principle of the MTT assay and how this assay helps youdetermine the LC50 of drug X.what are some advantages to using latex particles instead of blood cells for IM assay?Nicotinamide Adenine Dinucleotide (NAD) Assay (SIGMA Kit MAK037) analysis of tissue samples initially requires: centrifugation at 13,000g for 10min to homogenise tissue. centrifugation of 20mg of tissue at 2,000rpm with Extraction Buffer. freezing and thawing for 2 cycles of 20min before addition of Extraction Buffer. homogenization of PBS washed tissue with Extraction Buffer.