The following is an HPLC separation of deuterated benzenes on a C18 column that 440 cm long. The mobile phase was 30% acetonitrile:70% water at 30oC.What is the mobile phase velocity?Find the retention factor k' for C6D6.Find the plate number and plate height for C6D6..Assuming that the peak widths for C6H5D and C6H6 are the same as that of C6D6., find the resolution for C6H5D and C6H6.Retention times for C6H5D and C6H6 are 193.3 and 194.3 respectively, Find the relative retention time and unadjusted relative retention time between C6H5D and C6H6. Absorbance at 210 nm →→Thiourea(unretained)t = 41.7 minH₂NSNH2188.1DminDDD45DW1/2=1.01min2АлAt=1.01min185190195Time (min)54100200Time (min)D300CD3CD3 CH378D

1. mobile phase velocity (<v>) = 10.552 cm/min 2. retention factor (k') of C6D6 is 3.511 3.…

Answered: Absorbance at 210 nm →→ Thiourea…

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

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The following is an HPLC separation of deuterated benzenes on a C18 column that 440 cm long. The mobile phase was 30% acetonitrile:70% water at 30^oC.
What is the mobile phase velocity?
Find the retention factor k' for C₆D₆.
Find the plate number and plate height for C₆D₆..
Assuming that the peak widths for C₆H₅D and C₆H₆ are the same as that of C₆D₆., find the resolution for C₆H₅D and C₆H₆.
Retention times for C₆H₅D and C₆H₆ are 193.3 and 194.3 respectively, Find the relative retention time and unadjusted relative retention time between C₆H₅D and C₆H₆.