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HPLC Detector signal Q3: The Figure shown a HPLC chromatogram of two compound .Calculate the following values using the data in this chromatogram. 06- a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 05 03 لحرية 02 0.1 تختبر تحليل الى 45.0 s void 180.0 s uracil 247.5s guanine

HPLC Detector signal Q3: The Figure shown a HPLC chromatogram of two compound .Calculate the following values using the data in this chromatogram. 06- a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. c. The retention factor for uracil and guanine. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 05 03 لحرية 02 0.1 تختبر تحليل الى 45.0 s void 180.0 s uracil 247.5s guanine

Chemistry
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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please solve parts d and e and f, last three parts and thanks alot 

كرة HPLC Q3: The Figure shown a HPLC chromatogram of two compound .Calculate the following values using the data in this chromatogram. Detector signal a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. The retention factor for uracil and guanine. C. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 0.6- 05 04 03 0.2 0.1- 45.0 s vold A 15 30 45 تختبر تحليل الى 180.0 s Auracil 90 60 76 2475s guanine 105-120 135 150 166 180 195 210 225 240 255 270 296 Time, seconds
Transcribed Image Text:كرة HPLC Q3: The Figure shown a HPLC chromatogram of two compound .Calculate the following values using the data in this chromatogram. Detector signal a. The adjusted retention time of uracil and guanine. b. The peak widths for uracil and guanine. The retention factor for uracil and guanine. C. d. The selectivity factor for uracil and guanine. e. The resolution for these two compounds. f. The column efficiency parameters, N and H, if the column length is 10cm. 0.6- 05 04 03 0.2 0.1- 45.0 s vold A 15 30 45 تختبر تحليل الى 180.0 s Auracil 90 60 76 2475s guanine 105-120 135 150 166 180 195 210 225 240 255 270 296 Time, seconds
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