PART A: Substance A was found to have a retention times of 3.15 minutes on a 70.00-cm column. An unretained species passed through the column in 0.50 minutes. The peak width (at base) for A was 0.60 minutes. Using this information, determine the following:   1. What is the retention factor for analyte A?   2. What is the plate count for this column?   3. What is the height equivalent of a theoretical plate?

Chemistry
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ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
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PART A: Substance A was found to have a retention times of 3.15 minutes on a 70.00-cm column. An unretained species passed through the column in 0.50 minutes. The peak width (at base) for A was 0.60 minutes. Using this information, determine the following:

 

1. What is the retention factor for analyte A?

 

2. What is the plate count for this column?

 

3. What is the height equivalent of a theoretical plate?

 

PART B: Match each statement with one of these terms.

 

A Partition coefficient

B Mobile phase

C Elution

D Longitudinal diffusion

E Stationary phase

F Eddy diffusion

G Retention factor

H Plate number

I Dead time

J Retention time

K Selectivity factor

L Column resolution

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