A yeast geneticist irradiates haploid cells of a strain that is an adenine-requiring auxotrophic mutant, caused by mutation of the gene ade1. Millions of the irradiated cells are plated on minimal medium, and a small number of cells divide and produce prototrophic colonies. These colonies are crossed individually with a wildtype strain. Two types of results are obtained:(1) prototroph × wild type : progeny all prototrophic(2) prototroph × wild type : progeny 75% prototrophic, 25% adenine-requiring auxotrophsa. Explain the difference between these two types of results.b. Write the genotypes of the prototrophs in each case.c. What progeny phenotypes and ratios do you predict from crossing a prototroph of type 2 by the original ade1auxotroph?

Q: Alleles of genes A and B were analyzed in Neurospora according to the cross shown below. Ordered…

A: The four haploid cells are produced by the process of meiosis and three kinds of patterns of growth…

Q: DNA from a strain with genotype a+b+c+d+ is used to transform a strain with a genotype a-b-c-d-.…

A: In case of prokaryotic organisms such as bacteria sexual reproduction is achieved by horizontal gene…

Q: BACTERIA STRAIN A IS AUXOTROPHIC FOR METHIONINE AND STRAIN B IS AUXOTROPHIC FOR LEUCINE. A. WILL…

A: Auxotrophic strains lack the property of synthesising the growth factors required for their proper…

Q: State the types of crossovers that take part in Hfr × Frecombination. How do these crossovers…

A: Bacterial conjugation is the method of transit of genetic material amidst bacterial cells.

Q: Transgenic tobacco plants were obtained in which the vector Ti plasmid was designed to insert the…

A: A gene is a unit of hereditary present in thousands of numbers on the helical strands of…

Q: DNA from a strain of Bacillus subtilis with genotype a + b + c + d + e + is used to transform a…

A: Introduction :- The basic physical and functional unit of heredity is the gene. DNA is the material…

Q: Five independently isolated mutants of yeast all of which are unable to grow in the absence of…

A: The term genetic complementation of two genes suggests that the two mutated genes have a mutation at…

Q: Crosses are carried out between a bacterial F- strain and three different Hfr strains. In these…

A: The interrupted mating experiment is given in the question. It is used to find the order of genes…

Q: In the sample data from Sordaria shown in the picture: Count the total number of first and second…

A: the total number of crossing over and the total number of non-crossing over indicates the total…

Q: ARVCS is a disorder characterized by the replacement of healthy heart tissue with fatty fibrous…

A: Mutation is any change in the nucleotide sequence of the gene. It may lead to disorders if it makes…

Q: Bacteriophage λ, after infecting a cell, can integrate into the chromosome of the cell if the…

A: Temperate phages are bacterial viruses that as a part of their life cycle reside within the…

Q: Imagine that you have done a cross between two strains of yeast, one of which has the genotype A B C…

A: The scoring patterns in both tetrads indicate that recombination has occurred between genes A and C,…

Q: Which sample carries a derived X chromosome with a translocation due to ectopic crossover between…

A: In humans, sex is determined based on the presence or absence of sex chromosomes called the " X"…

Q: An Hfr strain of E. coli that is pro thr * leu * lac " was "mated" with an F- strain that is pro thr…

A: Introduction Genetic recombination is a process of exchange of genetic material between two…

Q: E. coli strains diploid for the lac region were constructed by introducing a plasmid carrying the…

A: ANSWER;- I- mutation- repressor is unable to bind to the operator region. If there is no other…

Q: In Neurospora, a mutant strain called stopper (stp) arose spontaneously. Stopper showed erratic…

A: The genes are the hereditary unit of an organism which are passed on from the parental generation to…

Q: The yeast genome has class 1 elements (Ty1, Ty2, and so forth) but no class 2 elements. What is a…

A: Yeast has a very compact genome having 70 % of its DNA as exons, therefore it contains insufficient…

Q: In Figure 5-17, how many crossovers are required to produce a completely prototrophic exconjugant?

A: The cell division causes the formation of identical daughter cells from the parent cell. The types…

Q: An Hfr strain that is leuA+ and thiL+ was mixed with a strain thatis leuA− and thiL−. In the data…

A: Transfer of genes in prokaryotes occurs through horizontal transfer mechanisms such as transduction,…

Q: You've crossed two strains: (Hfr) leu+ his+ bio+ with (F-) leu-his-bio- . Interrupted mating studies…

A: In biology, genetic recombination is the process by which genetic material is exchanged between…

Q: What is the recombination frequency?

A: Escherichia coli B is known to be the permissive strain for rII mutants since they permit the growth…

Q: A normal appearing female infant was identified with a positive newborn screen, linked to chromosome…

A: Hereditary genetic disorders can be devastating for the family and these are screened as early as…

Q: 12) Draw a yeast knockout cassette. Label all required sequence features.  a) Draw the target…

A: Hi! As you have posted multiple questions and asked to provide with two knockout diagrams, we are…

Q: In yeast, LYS5, ADE1, and URA2 are genes required to synthesize lysine, adenine and uracil,…

A: Given:MATa lys5 ADE1 URA2 haploid mated with MATa LYS5 ade1 ura2 haploid.Resulting triply…

Q: Below is a partially filled in complementation table. Please answer the following questions. 1 2 3 4…

A: Note:- Since you have posted a question with multiple subparts, so we will be solving the first…

Q: An experimental assay for the blood-clotting protein called factorIX is available. A blood sample…

A: Factor IX is a blood clotting protein, which belongs to the family of peptidase Sl. It was found in…

Mitochondrial Mutations

A mitochondrion is a cellular organelle that serves as the site for cellular respiration and energy production (ATP or Adenosine Triphosphate). It is a double-membrane-bound organelle and is only present in eukaryotic cells.