A scientist seeks to synthesize a rare human protein in lab. To accomplish this, she utilizes  recombinant techniques to insert the DNA of a eukaryotic gene for the protein into bacterial  plasmids. These plasmids are transformed into bacteria for expression. She is disappointed to  discover that the gene product from the bacteria is not the correct protein. What corrective step  could she take in the procedure to fix this issue?

Q: You are studying a human gene, and try to express the protein in E. coli bacterial cells. To do…

A: Cloning is the process of creating a genetically identical copy of a biological entity. Cloning can…

Q: Using nucleotide letters, show the kind of cut that could be made on a DNA molecule to circularize…

A: A plasmid is a small, extrachromosomal DNA molecule within a cell that is different from the…

Q: What bacteria were used for the isolation of the three enzymes (HindIII, NdeI and PvuI)? What type…

A: HindIII  is isolated from Haemophilus influenzae . NdeI is an  isolated from Neisseria…

Q: You want to clone the Human Gene A into a plasmid for producing the Protein A in bacteria. GeneA…

A: Introduction :- A plasmid is a small, circular, double-stranded DNA molecule that is separate from…

Q: nion and burger lover, your friend would like to incorporate the cheems gene (“cheM”) from the rare…

A: Transformation is a process in which the original DNA is transformed by the addition of foreign DNA…

Q: A human gene was insterted into a plasmid and then produced by a bacterium. Unfortunately, the human…

A: Biotechnology is involved with the genetic modification of microorganisms or enzymes from organisms…

Q: List the major steps that would be required to clone the human insulin gene into a plasmid and to…

A: Gene cloning is the isolation of DNA (deoxyribonucleic acid) sequence or gene of interest and…

Q: plate your cells on a master plate without any antibiotics.  After colonies appear, you pick and…

A: Recombinant DNA technology is helpful for different gene related experiments.

Q: Ihsan is a biologist working with the genetics of a psychrophilic bacterium. He cloned an antifreeze…

A: A gene is the basic physical and the functional unit of heredity. They are made up of DNA. Some…

Q: A plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A: Introduction :- Recombinant DNA technology ( also known as genetic engineering) involves the use of…

Q: If I clone a complete eukaryotic gene, including the eukaryotic promoter region, ligate it into a…

A: Transformation is the process in which a bacterium takes up DNA from the environment, that has been…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: "Biotechnology" is the use of our knowledge of biological processes to the development of beneficial…

Q: During nucleic acid hybridization, the probe is labelled O for DNA stability O to increase…

A:

Q: Why are nucleic acid probes necessary to lyse cells containing plasmids in order to detect the…

A: Cell lysis is frequently the very first step in most purification techniques for nucleic acids,…

Q: If a researcher wants to use bacteria to make a eukaryotic protein, she should use a plasmid from:…

A: The recombinant DNA technology involves transfer of foreign DNA into an organism. We used this…

Q: You want to express the protein of a newly discovered human gene using E. coli, so you clone the…

A: Protein tags are most frequently used to purify proteins for which no protein-specific antibody…

Q: Jackson Wang is a biologist working with the genetics of a thermophilic bacterium. He cloned a heat…

A: Gene cloning is a frequent process in molecular biology labs where researchers make clones of a…

Q: plasmid has been modified such that it carries the gene for green fluorescent protein (GFP) under…

A: GFP protein is found in the photoorgans of Aequorea Victoria a jelly fish. It is a 28 kDa protein.…

Q: plasmid vector has two genes in it, a gene for streptomycin resistance (strR) and another for…

A:

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the…

A: Insulin is a natural peptide hormone produced by the beta cells of the pancreas in response to high…

Q: You want to produce mass amounts of oxytocin, a small peptide hormone produced by the pituitary…

A: The molecular cloning is a technique of production of transgenic organisms. Here one gene is…

Q: In producing genetically engineered human insulin in bacteria, why is it important to use the same…

A: In the production of human insulin by bacterium, E.coli, the human insulin gene is introduced into…

Q: propose a method with the help of which they can generate many copies of the gene.

A: The production of multiple copies of a gene from a single gene, this technique is called as DNA…

Q: A plasmid vector has two genes in it, a gene for penicillin resistance (penR) and another for…

A: The plasmid vector with the inserted gene of interest is called a recombinant plasmid. The process…

Q: You are cloning a gene called ice, which will help strawberry plants survive in cold weather, into a…

A: Other incorrect options:B. This technique involves introducing the ice gene directly into the T-DNA…

Q: After decades of work, Dr. Ricky M. isolated a small amount of attractase—an enzyme that produces a…

A: A gene is the essential physical and functional unit of heredity. Genes are comprised of DNA. A…

Q: "Plasmids are still the workhorses for many applicationsof recombinant DNA technology". Define the…

A: Introduction  Microorganisms such as bacteria, viruses, etc. are not just pathogenic and harmful but…

Q: Margaret has been given a plasmid containing her favorite gene, afg (Margaret’s favorite gene). The…

A: First, convert the amount of plasmid from micrograms (μg) to nanograms (ng) since the concentration…

Q: Design the main steps of cloning experiment.

A: Microbial enzymes have been employed in a wide range of sectors, including chemical, agricultural,…

Q: There may be more than one correct answer, select all that apply.  To clone genes, a plasmid vector…

A: Introduction  A plasmid vector is a small, circular piece of DNA that is used as a tool for…

Q: The human gene for hemophilia is on the X chromosome. Below is a pedigree from a family afflicted…

A: Hi, Thanks For Your Question.  Answer : Correct Option Is b (20%) Explanation :  P (III-7 Is…

Q: If you are a genetic engineer and you cloned your gene of interest in a plasmid and you want to know…

A: Answer. Blotting describes the immobilization of sample nucleic acids/proteins onto a solid support.…