A research group discovers a new version of happyase, which they call happyase*, that catalyzes the chemical reaction The researchers begin to characterize the enzyme. (a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 uM/s. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.)
- A research group discovers a new version of happyase, which they call happyase*, that catalyzes the
chemical reaction
The researchers begin to characterize the enzyme.
(a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 uM/s. Based on this experiment, what is the kcat for happyase*? (Include appropriate units.)
(b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 uM, the researchers find that V0 = 300 nM/s. What is the measured Km of happyase* for its substrate HAPPY? (Include appropriate units.)
(c) Further research shows that the purified happyase* used in the first two experiments was actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully removed from the happyase* preparation and the two experiments repeated, the measured Vmax in (a) is increased to 4.8 uM/s, and the measured Km in (b) is now 15 uM. Based on this information, can you figure out what type of inhibitor is ANGER? (Use table 6.9 at the end of the Enzymes slides to determine the type of inhibitor)
![1. A research group discovers a new version of haRryase, which they call happyase*, that
catalyzes the chemical reaction
HAPPY =
SAD
The researchers begin to characterize the enzyme.
(a) In the first experiment, with [Et] at 4 nM, they find that the Vmax is 1.6 uM/s. Based on this
experiment, what is the kcat for haRryase*? (Include appropriate units.)
(b) In another experiment, with [Et] at 1 nM and [HAPPY] at 30 uM, the researchers find that VO
= 300 nM/s. What is the measured Km of happyase* for its substrate HAPPY? (Include
appropriate units.)
(c) Further research shows that the purified happyase* used in the first two experiments was
actually contaminated with a reversible inhibitor called ANGER. When ANGER is carefully
removed from the happyase* preparation and the two experiments repeated, the measured
Vmax in (a) is increased to 4.8 uM/s, and the measured Km in (b) is now 15 uM. Based on this
information, can you figure out what type of inhibitor is ANGER? (Use table 6.9 at the end of
the Enzymes slides to determine the type of inhibitor)](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F17f01e62-6233-4011-be03-db79751c68ba%2F6c1c349e-794b-47c4-92bf-7c7ec3536e21%2Futny4vd_processed.png&w=3840&q=75)
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