A Meselson-Stahl-experiment was performed to study the DNA replication ofa newly discovered bacterium. This bacterium takes 30 min to complete a roundof replication at 37° C. Figure 2.1 shows the autoradiography of the replicatingDNA molecule.BACDFigure 2.1(i)Describe the result of this experiment.

J.Cairns used autoradiography to establish the semi-conservative nature of DNA replication and the…

Answered: A Meselson-Stahl-experiment was…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

See similar textbooks

Similar questions

Circle the section on the DNA template where the example primer would bind.
Use the gel to answer the following questions. You will be constructing a map of the plasmid, pDiddy. IF the EcoRI/BamHI double digest produces 3 fragments with only two sizes, what are their sizes? 2.5 kb, 250 b and 250 b 3.0 kb 2.0 kb 500 b and 500 b 3.0 kb 1.0kb and 1.0 kb
Choose the one answer that fits best. The pGLO plasmid you used contains an araC gene, a GFP gene and a bla gene. If you performed the exact same experiment with a plasmid that contained a GFP gene and a bla gene, but was missing the araC gene, what would you expect to see on your four plates? a. The positive control would not have any bacterial lawn b. The negative control would have as many colonies as the experimental plate c. The LB/Amp/Ara plate would have some colonies but they would not be fluorescent under UV light d. The experimental plate would not have any colonies e. The LB/Amp plate containing E. coli (+)pGLO would be fluorescent
Pictured below is a map of the pBR322 plasmid vector. If a DNA fragment is inserted into the Sal I site, what can be expected about the growth coli transformants containing the recombinant plasmid? EcoRI Pstl Ampicillin resistance (AmpⓇ) Origin of replication (ori) d. All of the above are true pBR322 (4,361 bp) Pvull BamHI Tetracycline resistance (TetⓇ) Sall a. The transformants should grow on nutrient agar containing ampicillin and tetracycline. b. The transformants should be able to grow on nutrient agar without antibiotics. c. The transformants should be able to grown on nutrient agar containing ampicillin
Which plate could represent the results of the transformation experiment positive control plated on LB if the transformation procedure was too harsh on the bacterial cells? Refer to the letters on top of the image. Ignore the letters at the bottom. B A O A OB O none of the choices are correct
You transform 500 uL of cells with 2.5 ug of plasmid DNA, and then you take 100 uL and spread that on a plate. 3 days later you count 11 cfu. What was the transformation efficiency? use the following formula: TE =(VP/VTT) * (cfu/ug of DNA)
There are 2 parts to this question: The following DNA strand (below) is about to undergo DNA replication. a) Please replicate the parental strands into two exact copies TC GATATCGG AGCTATAGCC b) place a centromere between the two replicated copies (or tell me where the centromere would be located),
Copy and paste the link below and watch the video on Youtube and Answer the Questionshttps://www.youtube.com/watch?v=g-dNJdOvBM4 Polymerase Chain Reaction Questions: 1. What are the materials used for the polymerase chain reaction? 2. Draw a schematic diagram of the procedure in PCR. 3. Why is it important to design the primers at the start of the laboratory procedure? 4. What are the components of the PCR buffer and what is its pH range? What is the purpose of the buffer? 5. What is the use for magnesium chloride? 6. How much template DNA is added? What is the concentration of the primers? 7. At what temperatures does denaturation, annealing and extension occur? Why are the processes placed in that temperature? 8. In this particular PCR experiment, how many cycles was used? 9. Can this PCR be used on its own to find out if a person has Covid or not on its own? Why or why not?
To test patients for COVID19, lab workers will first convert all the RNA molecules extracted from a nasal swab to a double-stranded DNA copy (dsDNA). If the virus is present, its genomic sequence should be in some of the new dsDNA molecules. Part 1) A region of COVID genomic DNA sequence is shown below. Following convention, only the top strand is shown. Copy/paste the sequence into the text box and create the second strand. Be sure to label its ends. (You may need to reduce the font size so that it doesn't wrap around) AAGATCACATTGGCACCCGCAATCCTGCTAACAATGCTGCAATCGTGCTACAACTTCCTC Part 2) To test for the presence of COVID DNA sequence, lab workers use single-stranded DNA oligonucleotides as probes (short pieces of DNA that do not have a partner strand). If the two strands of DNA that you drew were separated from each other, where would the shorter DNA strand shown below be able to form continuous base pairs? Highlight that region in your dsDNA model. TGTAGCACGATTGCAGCATTG Note: If you…
they decided to check which bacterial colonies contains the recombinant gene W by PCR. they designed one set of primers, gene W forward, and gene W reverse, to amplify gene W. Complete the PCR profile that will be used below.
Perform the Megaprimer PCR procedure (in scheme A). Indicates the number of Primers required in the protocol and marks their location according to the location of the nucleotide to be mutated (the X represents the nucleotide to be mutated). A)
Can you please answer this question

SEE MORE QUESTIONS

Recommended textbooks for you

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781319114671

Author:

Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:

W. H. Freeman

Lehninger Principles of Biochemistry

Biochemistry

ISBN:

9781464126116

Author:

David L. Nelson, Michael M. Cox

Publisher:

W. H. Freeman

Fundamentals of Biochemistry: Life at the Molecul…

Biochemistry

ISBN:

9781118918401

Author:

Donald Voet, Judith G. Voet, Charlotte W. Pratt

Publisher:

WILEY

Biochemistry

ISBN:

9781305961135

Author:

Mary K. Campbell, Shawn O. Farrell, Owen M. McDougal

Publisher:

Cengage Learning

Biochemistry

ISBN:

9781305577206

Author:

Reginald H. Garrett, Charles M. Grisham

Publisher:

Cengage Learning

Fundamentals of General, Organic, and Biological …

Biochemistry

ISBN:

9780134015187

Author:

John E. McMurry, David S. Ballantine, Carl A. Hoeger, Virginia E. Peterson

Publisher:

PEARSON

SEE MORE TEXTBOOKS