A bloactlve hexapeptide was cleaved by trypsln from a larger proteln. The peptlde wassequenced by treatment with varlous reagents and enzymes. The results are as follows:a) Treatment wlth cyanOgen bromlde gave a tetrapeptlde of compositlon asp, trp, cysand met.b) Treatment with chymotrypsln gave a tripeptlde of compositlon asp, trp and cys, adipeptlde wlth the same C-term and an amlno acld.c) Treatment with streptococcal protease gave a dipeptlde and a tetrapeptlde.The composltion of the peptlde Is lys, trp, asp, cys and met1. What Is the sequence of the hexapeptlde?2. What is the pl of thls peptlde?

Proteins are the polymers of amino acids. These are also known as polypeptides. Hexapeptide is an…

Answered: A bloactlve hexapeptide was cleaved by…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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What is the principle behind the very specific sequence in donning and doffing PPEs? Explain
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A number of yeast-derived elements were added to thecircular bacterial plasmid pBR322. Yeast that requireuracil for growth (Ura− cells) were transformed withthese modified plasmids and Ura+ colonies were selected by growth in media lacking uracil. For plasmidscontaining each of the elements listed in parts (a) to(c), indicate whether you expect the plasmid to integrate into a chromosome by recombination, or insteadwhether it is maintained separately as a plasmid. If theplasmid is maintained autonomously, is it stably inherited by all of the daughter cells of subsequent generations when you no longer select for Ura+ cells (that is,when you grow the yeast in media containing uracil)?a. URA+ geneb. URA+ gene, ARS c. URA+ gene, ARS, CEN (centromere)d. What would need to be added in order for these sequences to be maintained stably in yeast cells as alinear artificial chromosome?
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1) In MALDI-TOF data, when performing peptide mass mapping search, a) The number of peptides matched decreases from 13 to 11 and the sequence coverage decreases to 47%, but the protein score increases from 111 to 124. Explain. b) The nnumber of 11 peptide masses matched and the sequence coverage is 47% (same as in part a), but the protein score increases to 157. Explain.
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