(a) As the pH changes from 9.0 to 5.0, how does the concentration of H+ (protons) change? (b) Describe how the charge of some amino groups in a protein might differ at pH 9.0 and pH 5.0. (c) Describe how the charge of some carboxyl groups in a protein might differ at pH 9.0 and pH 5.0. (d) Given your answers to parts (b) and (c), what kind of intramolecular interactions in beta-galactosidase are most likely to be affected by a change in pH from 9.0 to 5.0? (e) Could the interactions you mention in part (d) affect the catalytic activity of beta-galactosidase?
Nucleotides
It is an organic molecule made up of three basic components- a nitrogenous base, phosphate,and pentose sugar. The nucleotides are important for metabolic reactions andthe formation of DNA (deoxyribonucleic acid) and RNA (ribonucleic acid).
Nucleic Acids
Nucleic acids are essential biomolecules present in prokaryotic and eukaryotic cells and viruses. They carry the genetic information for the synthesis of proteins and cellular replication. The nucleic acids are of two types: deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The structure of all proteins and ultimately every biomolecule and cellular component is a product of information encoded in the sequence of nucleic acids. Parts of a DNA molecule containing the information needed to synthesize a protein or an RNA are genes. Nucleic acids can store and transmit genetic information from one generation to the next, fundamental to any life form.
(a) As the pH changes from 9.0 to 5.0, how does the concentration of H+ (protons) change?
(b) Describe how the charge of some amino groups in a protein might differ at pH 9.0 and pH 5.0.
(c) Describe how the charge of some carboxyl groups in a protein might differ at pH 9.0 and pH 5.0.
(d) Given your answers to parts (b) and (c), what kind of intramolecular interactions in beta-galactosidase are most likely to be affected by a change in pH from 9.0 to 5.0?
(e) Could the interactions you mention in part (d) affect the catalytic activity of beta-galactosidase?
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