A 1/10 dilution of enzyme extract has been done for the protein assay. The experimental results are given below. Tube Phosphate buffer 0.1 M pH 7 (µL) Diluted enzyme extract (μL) Bradford reagent (mL) DO at 595 nm 0,8 0,7 0,6 0,5 0,4 0,3 0,2 0,1 0 A595 1- Calculate the mass of proteins contained in 50 or 100 μL of diluted extract thanks to the calibration curve (graph below), performed with serum albumin (SAB). 2- Calculate the protein concentration of undiluted enzyme extract. 0 1 50 50 1 0.102 2 3 4 5 50 0 0 50 100 100 1 1 1 0.098 0.101 0.199 0.200 4 2 50 6 8 10 SAB (ug per tube) Data: the slope of the calibration curve is 0,05 µg¹. 50 1 12 14 6 0 100 1 0.200 16
A 1/10 dilution of enzyme extract has been done for the protein assay. The experimental results are given below. Tube Phosphate buffer 0.1 M pH 7 (µL) Diluted enzyme extract (μL) Bradford reagent (mL) DO at 595 nm 0,8 0,7 0,6 0,5 0,4 0,3 0,2 0,1 0 A595 1- Calculate the mass of proteins contained in 50 or 100 μL of diluted extract thanks to the calibration curve (graph below), performed with serum albumin (SAB). 2- Calculate the protein concentration of undiluted enzyme extract. 0 1 50 50 1 0.102 2 3 4 5 50 0 0 50 100 100 1 1 1 0.098 0.101 0.199 0.200 4 2 50 6 8 10 SAB (ug per tube) Data: the slope of the calibration curve is 0,05 µg¹. 50 1 12 14 6 0 100 1 0.200 16
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Transcribed Image Text:A 1/10 dilution of enzyme extract has been done for the protein assay. The
experimental results are given below.
Tube
Phosphate buffer 0.1 M pH 7 (µL)
Diluted enzyme extract (uL)
Bradford reagent (mL)
DO at 595 nm
0,8
0,7
0,6
0,5
0,4
0,3
0,2
0,1
A595
0-
0
1
50
50
1
0.102
1- Calculate the mass of proteins contained in 50 or 100 μL of diluted extract thanks to the
calibration curve (graph below), performed with serum albumin (SAB).
2- Calculate the protein concentration of undiluted enzyme extract.
2
3
5
50
50
0
50
50
100
100
1
1
1
1
0.098 0.101 0.199 0.200
2
6
8
10
SAB (ug per tube)
Data: the slope of the calibration curve is 0,05 µg¹¹.
4
0
12
14
6
0
100
1
0.200
16
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