9. Here is a restriction map for a bacterial plasmid showing the cleavage sites for two different restriction enzymes EcoRI and Bam H1. EcoR1 700 bp EcoR1 2000 bp 1200 bp BamH1 Match the banding pattern in each lane of the agarose gel below with one of the treatments listed. Note: Not all treatments listed are run on the gel. Plasmid treatments: A. BamHI+EcoR1 (no RNAse) B. BamHI only (no RNAse) Gel lanes: 1. DNA Ladder = Mol. Wt. Standard (sizes indicated) bp 5000 4000 3000 2000 1650 1000 850 650 500 C. BamH1 + EcoR1 + RNAse D. RNAse only 2. E. BamH1 + RNAse F. EcoR1+ RNAse 3. G. EcoR1 only (no RNase) 4. 1 2 3 4 5 6 5. 6. -

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
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Chapter17: Bacterial And Viral Genetics
Section: Chapter Questions
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9. Here is a restriction map for a bacterial plasmid showing the cleavage sites for two different restriction
enzymes EcoRI and Bam H1.
EcoR1
700 bp
EcoR1
2000 bp
1200 bp
BamH1
Match the banding pattern in each lane of the agarose gel below with one of the treatments listed. Note: Not all
treatments listed are run on the gel.
Plasmid treatments:
A. BamHI+EcoR1 (no RNAse)
B. BamHI only (no RNAse)
Gel lanes:
1. DNA Ladder = Mol. Wt. Standard
(sizes indicated)
bp
5000
4000
3000
2000
1650
1000
850
650
500
C. BamH1 + EcoR1 + RNAse
D. RNAse only
2.
E. BamH1 + RNAse
F. EcoR1+ RNAse
3.
G. EcoR1 only (no RNase)
4.
1 2 3 4
5
6
5.
6.
-
Transcribed Image Text:9. Here is a restriction map for a bacterial plasmid showing the cleavage sites for two different restriction enzymes EcoRI and Bam H1. EcoR1 700 bp EcoR1 2000 bp 1200 bp BamH1 Match the banding pattern in each lane of the agarose gel below with one of the treatments listed. Note: Not all treatments listed are run on the gel. Plasmid treatments: A. BamHI+EcoR1 (no RNAse) B. BamHI only (no RNAse) Gel lanes: 1. DNA Ladder = Mol. Wt. Standard (sizes indicated) bp 5000 4000 3000 2000 1650 1000 850 650 500 C. BamH1 + EcoR1 + RNAse D. RNAse only 2. E. BamH1 + RNAse F. EcoR1+ RNAse 3. G. EcoR1 only (no RNase) 4. 1 2 3 4 5 6 5. 6. -
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