9. For the DNA segment with nucleotide sequence 5' TATCGC 3', how many hydrogen bonds in total hold the base pairs between this segment and its complementary strand? А. 5 В. 6 С. 15 D. 18 E. Cannot be determined 10. What is the relation between the Fischer projection of two molecules below? H- HO- Но

9. For the DNA segment with nucleotide sequence 5' TATCGC 3', how many hydrogen bonds in total hold the base pairs between this segment and its complementary strand? А. 5 В. 6 С. 15 D. 18 E. Cannot be determined 10. What is the relation between the Fischer projection of two molecules below? H- HO- Но

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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The cationic polymerization of a-methylstyrene can be initiated by the BCI3/H2O initiating system. Assume the termination reaction occurs only by chain transfer to monomer by b-proton transfer. Draw the complete chemical structure for a poly(a-methylstyrene) containing three a-methylstyrene repeat units that results from this polymerization. 05 10:06 AM
1. What is the name of the nucleobase in the nucleotide structure? a. guanineb. adeninec. thymined. uracil 2. Is the nucleobase a purine or a pyrimidine?
33. Gel filtration chromatography shows that the molecular weight of a protein is 240,000 Daltons. However, when the protein was subjected to SDS-PAGE in the absence of ß- mercaptoethanol, two polypeptides (100,000 Daltons, 140,000 Daltons) were identified. Furthermore, when the protein was subjected to SDS-PAGE in the presence of ß- mercaptoethanol, three polypeptides (100,000 Daltons, 90,000 Daltons, and 50,000 Daltons) were identified. Which of the following statements is true of the protein? A. The protein is composed of three polypeptides. B. The three-dimensional structure of the protein is stabilized by both covalent and non- covalent bonds. C. Both A and B. D. Neither A nor B. 34. What is purpose of SDS in SDS-PAGE? E. To selectively bind the target protein F. To maintain buffer pH in the gel G. To cause the separation of proteins to be on the basis of molecular weight only H. To initiate polymerization of acrylamide to form gel
1. Amino Acid 1 = W + X = 1 Draw amino acid 1 at a low pH a) As the D Fisher Projection Name of amino acid 1: b) As the S isomer: Arginine
24. Answer and explain
10. A new protein of unknown structure has been purified. Gel filtration chromatography reveals that the physiological protein has a molecular weight of 240,000 Daltons. Chromatography in the presence of 6 M urea yields a single peak corresponding to a protein of 60,000 Daltons. Chromatography in the presence of 6 M urea and 10 mM mercaptoethanol yields peaks for proteins of 34,000 Daltons and 26,000 Daltons. What can we learn about protein's tertiary and quaternary structure from this data?
7. Hydrogen bonds are a crucial component of biomolecular structure and biomolecular interactions. In the following drawings, draw in several hydrogen bonds that are possible between atoms within the same molecule or between different molecules: A: Protein structure H₂C₁ #1 N-H N-C 0=C HR 1 N-H ORU 0=C N-HH-CR B: DNA base-pairing H H-N N-H N=C C-C H 0=C NIH C C=N HR 0=3₁ HR H-9-4 0-0 NIH N-H 0=C 0=0 RECH N-H N-H 0=8-₁ OH HR C: Plant cell wall (cellulose) CH₂OH H BỆNH O=C HA CH OH H CH₂OH ( он H HC-0 CH₂₂OH H OH 1 TH OH H IN HC- OH H CH₂OH CH₂₂OH O OH H C-CH H bH CH₂₂OH VA HS COK H ОН Biomolecules like DNA, proteins, and carbohydrates depend on hydrogen bonds to maintain their overall structure. However, each individual hydrogen bond is relatively weak. Using protein structure from the question above as an example, explain how to resolve the apparent contradiction between these two statements.
9.2
Double stranded nucleic acids are stabilized by all of the following except: A. Hydrophobic stacking between bases B. Hydrogen bonding between pyrimidines and purines C. Repulsion of phosphate groups D. Phosphodiester backbones E. Hydrogen bonding via ether linkages
1) Describe one inner and one outer approach for the prediction of contact or distance maps in proteins.
2. | Calculate the overall charge (pH 7) on the following three polypeptides and answer the questions below. Assume the following pKa values: N-terminal –NH3®, 7.0; all -COOH groups, 4.0; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. (A) Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys–Pro–Val–Gly–Lys–Lys–Arg-Arg-Pro-Val-Lys–Val-Tyr-Pro-Asp-Ala -Gly- Glu-Asp-GIn– Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe (B) Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly-Glu-Glu–Cys-Asp-Pro-Val-Glu–Val–Tyr-Pro-Asp- Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu-Phe-Cys-Ser-Tyr-Ser-Met-Glu–His-Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val–Gly–Glu-Glu-Cys-Asp-Pro–Val-Glu–Val–Tyr-Pro-Asp-Ala (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu-Val-Tyr-Pro-Asp- Lys–Ala-Gly-Arg-Glu-Ser-Arg-Ala (d) Which of the three peptides would migrate the closest to the anode in isoelectric focusing? (e) Which of the above peptides would elute last from a gel filtration column? (f) Which of the three…
H2N N. OH H2N NH" NH, Но H) 18. Name of base A: 19. Name of base D: 20. Base pair name of molecule A: 21. Base pair name of molecule D: 22. Name of molecule in box E: 23. Name of molecule in box F: 24. G is the end: 25. H is the end: