45-year-old male had been working in his garden when he scratched the knuckle of his right arm during digging in ground with a spade, he immediately washed the area but noticed later that night that the knuckle became red, warm to the touch and swollen. He went to the hospital, the doctor ordered a Gram stain and culture on the pus extracted from the wound. The culture grew the following organism and the Gram stain from the wound showed the following results: Based on the previous results, answer the following questions: 1. As a microbiologist, describe what you see on the Gram stain taken from the wound site. And give a preliminary report on what you see on the culture plates. What is the probable Genus and species? 2. Name 2 tests that can be done to positively identify this organism. 3. What are the media used to culture the sample, and what is the type of hemolysis seen?

Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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45-year-old male had been working in his garden when he scratched the knuckle of his right arm during digging in ground with a spade, he immediately washed the area but noticed later that night that the knuckle became red, warm to the touch and swollen. He went to the hospital, the doctor ordered a Gram stain and culture on the pus extracted from the wound. The culture grew the following organism and the Gram stain from the wound showed the following results:

Based on the previous results, answer the following questions:
1. As a microbiologist, describe what you see on the Gram stain taken from the wound site. And give a preliminary report on what you see on the culture plates. What is the probable Genus and species?
2. Name 2 tests that can be done to positively identify this organism.
3. What are the media used to culture the sample, and what is the type of
hemolysis seen?

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