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Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

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Gram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....

Expert Solution

Step 1

On the basis of gram staining, we can differentiate between two types of bacteria, one with thick cell wall and one with thinner one.

The procedure of gram stain is as follows:

First step is staining with crystal violet stain which is chemically hexamethyl-para-rosaniline chloride.
Then a decoloriser, ethyl alcohol or acetone is added. In gram positive bacteria peptidoglycan layer of cell wall is thick so, gram stain is taken and is not decolorised but in gram negative bacteria peptidoglycan layer of cell wall is thin, so it is decolorised.
Afterwards, a counter stain usually safranin is added, that gives orangish to pink color to gram negative bacteria while gram positive remains purple.