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Medical Terminology for Health Professions, Spiral bound Version (MindTap Course List)

8th Edition

ISBN:9781305634350

Author:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder

Publisher:Ann Ehrlich, Carol L. Schroeder, Laura Ehrlich, Katrina A. Schroeder

Chapter3: The Skeletal System

Section: Chapter Questions

Problem 42LE: CR __________________________________

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Question

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Gram straining procedure. Indicate the following information A. Shape........ B. Stained color under microscope........ C. PG Wall thick, thin or both.....

Expert Solution

Step 1

On the basis of gram staining, we can differentiate between two types of bacteria, one with thick cell wall and one with thinner one.

The procedure of gram stain is as follows:

First step is staining with crystal violet stain which is chemically hexamethyl-para-rosaniline chloride.
Then a decoloriser, ethyl alcohol or acetone is added. In gram positive bacteria peptidoglycan layer of cell wall is thick so, gram stain is taken and is not decolorised but in gram negative bacteria peptidoglycan layer of cell wall is thin, so it is decolorised.
Afterwards, a counter stain usually safranin is added, that gives orangish to pink color to gram negative bacteria while gram positive remains purple.