A researcher is planning to plant antibiotic-resistant Escherichia coli pure bacteria sample in antibiotic (ampicillin) solid media in his experiment. Describe each of the sterilization methods that this researcher should use while preparing this solid medium with antibiotics and in cultivation of the bacteria on the solid medium, indicating the stages at which it should be used and giving the mechanism of action.

Q: There are two bacteria, and I have to find out the organism. I did gram staining, and I found out…

A: Gram staining is a laboratory technique used to differentiate and classify bacteria into two major…

Q: Antibiotics should be reduced or eliminated from culture media during cell subculturing. Make it…

A: Subculturing, also known as passaging cells, is the process of removing the media from an old…

Q: What is the principle of freezing of cell lines and primary cells.Describe the general protocol for…

A: Cell culture is a procedure in which cells (either animal or plant cells) are separated from an…

Q: What is the purpose of flaming an inoculating loop?  How will you know when you have flamed the loop…

A: Hi, since you have posted multiple questions, we will be providing you with answers for the first…

Q: Isolating a pure culture from a mix sample is typically done on solid agar but can be performed…

A: A microbial culture is a method of multiplying microbial organisms by letting them reproduce in…

Q: You are interested in obtaining Staphylococcus aureus for a study investigating the prevalence of…

A: S.Aureus is the most common cause of abscesses, furuncles, and cellulitis in the skin and soft…

Q: Why is CFU more applicable to a culture of streptococcus than to a culture of E.coli

A: Streptococcus is a Gram-positive bacteria that are spherical in shape. This bacteria is responsible…

Q: Using one of the graphs obtained with the medium II, name the different phases of the bacterial…

A: Micro organisms are typically grown in a closed culture media. Nutrients are added according to the…

Q: Why is the effectiveness of an antimicrobial agent determined using an exponentially growing…

A: The bacterial cell reproduce asexually by replicating and dividing their genetic material as well as…

Q: Are the large numbers of microorganisms found in the mouth cause for concern? Explain.

A: We are authorized to answer one question at a time.Since you have not mentioned which one,I am…

Q: A bioreactor which is used for the production of growth factor A is operated as fed- batch culture.…

A: A Bioreactor is a device or a process system which supports and habitats the biologically active…

Q: Samples from an ill patient were collected by a physician for testing. A bacterial infection is…

A: Infections are the due to the activity of microbes in the body. Microbes can colonize in human body.…

Q: Lactose fermentation

A: The dichotomous key is the tool for the identification of unknown organism among the group. Choice…

Q: Why is it important for a microbiology laboratory technician to use aseptic technique?

A: The majority of the microbes onto which scientists work in laboratories are known pathogens.

Q: Describe how specimen collection is performed using aseptic techniques to isolate medically…

A: INTRODUCTION Escherichia coli It is an aerobe and facultative anaerobe garm negative bacilli forms…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: You inoculate a species of bacteria on a MacConkey agar which normally shows positive growth and the…

A: MacConkey Agar (MAC) is selective and differential agar that is used to isolate non-fastidious…

Q: What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate

A: The Agar plate is made from mixing agarose and a growth medium onto a petri dish. Agarose is in a…

Q: You must find up your OWN dichotomous key for all the possible unknown organisms listed below. The…

A:

Q: procedure/s in performing aseptic transfer of bacterial cultures in (include illustration) (3) agar…

A: Aseptic Technique describe some of the ways that a laboratory can deal with the constant threat of…

Q: Amikacin Antimicrobials Amoxicillin/clavulanate Ampicillin Cefazolin Cefepime Ceftriaxone…

A: Excessive Zn++ in the medium can have various effects on different groups of living organisms.…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of…

A: The bacteria in the laboratory grow under appropriate biochemical and biophysical environments. The…

Q: Describe how each of the following would appear when grown on EMB agar: a. Escherichia coli b.…

A: EMB stands for Eosin Methylene Blue. This media is used as a seletive stain for the gram negative…

Q: What would happen if an ampicillin reagent is added to an Lysogeny broth agar plate that is below 50…

A: Ampicillin is an antibiotic which enables the synthesis of the peptidoglycan of bacterial cells wall…

Q: For a laboratory exercise of basic techniques in microbiology: Gram stain and Microscopy. What are…

A: "Gram staining is important in microbiology because it allows scientists to quickly and easily…

Q: What are the possible causes of having Too Numerous To Count (TNTC) bacterial colonies in an agar…

A: Introduction Bacteria is a prokaryotic, unicellular organism. They belong to the kingdom Monera.…

Q: Why is heat-fixed procedure in bacterial smear preparation not ideal for capsular staining?

A: In microscopic studies, the specimen needs to be prepared for an optimized vision under a…

Q: Explain why sulfa drugs prevent bacterial growth without harming the human host.

A: Antimicrobial drugs are those which destroy or inhibit the growth of microorganism. They have their…

Q: one or your lab partners has followed the rrecommended procedure of running Gram-positive and Gram…

A: Gram staining is a common laboratory test to characterize the bacteria into gram-positive and…

Q: LB (Lysogeny broth) medium for the growth of E. coli,

A: Pseudomonas putida is a kind of rod-shaped, Gram-negative, non-fermenting bacterium which is present…

Q: Demonstrate your understanding of industrial microbiology by explaining the involvement of in the…

A: Industrial microbiology is a well known field of microbiology in which various microorganisms are…

Q: Remove the agar plate from its lid with the left hand. While holding the plate, inoculate the agar…

A: Introduction -- Microbes are around and every where in environment. Sterile agar plates which…

Q: What is an E-test strip, and what is it used for in the microbiology laboratory? Draw a diagram of…

A: Microbiology is a branch of science which deals with the study of microscopic organisms such as…

Q: Identify the oxidase-negative, gram negative bacillus isolated from the blood cultures of a…

A: Gram-negative bacteria are those that do not retain the crystal violet stain after gram staining.…

Q: Refer to the provided image drawn by a student trying to plan out their serial dilution protocol.…

A: Colony forming unit (CFU)is a measure of viable bacterial cell. CFU/ml can be calculated using…

Q: Identification of Gram-positive cocci: You have isolated a catalase-negative bacterium that doesn't…

A: * CAMP test ala called as Christie Atkins Munch peterson test which is used to identify group B…

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: You were given a mixed nutrient agar broth culture of bacteria  1a. How will you determine the…

A: A mixed bacterial culture consists of more than one bacterial cell types growing together. On the…

Q: e the process to make a 4-phase streak plate beginning with your first streak (i.e you have…

A: The sample/inoculum is usually pored by streak with the diluted through streaking it across the…

Modes of Nutrition

It is a biological process of providing or obtaining food that is necessary for growth and health. It includes many physiological processes - absorption, ingestion, assimilation, catabolism, excretion, etc. In biology, the science that deals with nutrition's physiological and chemical processes are called "nutritional science."