Given the scenario, compute for the total volume of the culture media solution (milliliter or liter) and dehydrated media (grams). Scenario: The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter   Formula: C1V1 = C2V2 *Concentration *Volume   Computation: What are the answers to the following. Weight in grams of nutrient broth: _________ Distilled water in mL for nutrient broth: __________ Weight in grams of nutrient agar __________ Distilled water in mL for nutrient agar: ____________

Q: Make a flowchart for the procedure of SPREAD PLATE METHOD

A: The spread plate method is a method to plate a fluid sample containing bacteria with the goal that…

Q: Calculate the CFU/ml of leuconostoc in the brine for the 4 different days. Show your calculation

A: CFU/ml stands for "colony forming units per milliliter." It is a unit of measurement used in…

Q: One ml of a water sample was placed in a Petri dish with melted agar. After incubation, 129 colonies…

A: A colony-forming unit, or CFU, is a measurement of the concentration of microorganisms in a test…

Q: A swab for culture is received from a doctor's  office. The staff forgot to mention the source from…

A: Introduction Microbiology is the branch of science that deals with the study of different types of…

Q: How would you draw out a step in an experiment that included the following? Please help, I am so…

A: Horizontal gene transfer (HGT) is largely responsible worldwide for increasing the incidence of…

Q: transferred to the plate. After 24 hours there were 60 colonies on the plate. What is the dilution…

A: b. The dilution factor is 1000. The patient sample contains 600,000 cells/ml

Q: The number of bacteria in the samples need to be quantified. The laboratory uses a specific protocol…

A: Introduction :- Bacteria are single-celled organisms with a small size. Bacteria can be found…

Q: Describe characteristics of Proteus Vulgaris in the Agar: Blood Agar MacConkey Agar EMB PEA Mannitol…

A: Non-human bacteria, viruses, and fungi outweigh human cells, making our body a home and source for a…

Q: Lactose fermentation

A: The dichotomous key is the tool for the identification of unknown organism among the group. Choice…

Q: he image below is the results of the Nutrient agar plate inoculated with the kitchen ounter sample…

A: Colony morphology is used by microbiologists to identify and characterized the different bacterial…

Q: Describe how you would execute this first stage from the point you are handed the nutrient broth…

A: For isolation of any culture, it needs to be incubated in broth to obtain full fledged turbidity or…

Q: MacConkey agar inhibits the growth of Gram-positive bacteria while encouraging the growth of…

A: In microbiology, MacConkey agar is a versatile medium that is frequently used. It is a selective…

Q: ou've been tasked with observing the motility of an UNKNOWN Bacteria isolated from a mixed culture.…

A: Bacteria is the tiny cellular living structure which belong to the  Moneran kingdom. The bacteria…

Q: identify the organisms in  an unknown culture. You find that the culture contains two gramnegative…

A: A culture is a medium to grow bacteria and other microorganisms  It provides the bacteria with…

Q: a. Explain whether or not any of the methods in fi gure 2.9 could beused to determine the total…

A: Since, the question a. in incomplete as it requires figure 2.9 to be answered we will answer the…

Q: discuss the significance of the different aseptic techniques utilized in maintaining sterility of…

A: The laboratory that carries out growth of prokaryotic cells or eukaryotic cells in physiological…

Q: Drawings of the two bacterial cultures as observed under the 4 mm objective. Describe features…

A: Staphylococcus aureus and Streptococcus pyogenes are gram positive bacteria that cause infections in…

Q: Which type(s) of medium would be used in each scenario?a. isolating the growth of Streptococcus…

A: A culture medium, also called the growth medium, can be a liquid or solid medium that supports the…

Q: As shown in this diagram, you perform a ten-fold serial dilution of a culture to determine the…

A: The number of microorganisms that are able to divide and form new colonies is called a…

Q: 3) Define gel electrophoresis, including its theory and application. Describe the steps of running…

A: Introduction:- Electrophoresis is a biophysical technique mainly used to separate proteins and…

Q: Please use this submission link to submit your flowcharts. You will need to submit a total of three…

A: Microbial detection is necessary for the identification of microbes, especially bacteria. They spoil…

Q: Please create a dichotomous key using these tests to isolate each bacteria strain.

A: The microorganisms distinguished by many methods and gram staining is one of the most important…

Q: Based on these results, which of the following statements is true regarding the effectiveness of…

A: A disinfectant is a chemical substance or compound that is used to inactivate or destroy the…

Q: If you were using the quadrant streak plate method to plate a very dilute broth culture ( with many…

A: Quadrant streaking is a microbiological technique which is used for isolation of pure strain from…

Q: Why is it best to use sterile distilled water in the preparation of microbial suspension and dry…

A: Smear preparation is the first step carried out during the staining procedure.

Q: A patient sample will be analyzed. You are responsible to quantify the bacteria number. The dilution…

A: A sample of your blood, urine, skin, or any part of your body will be taken during a bacteria…

Q: How could calculate this serial dilutions probelms for microbiology and how do can I know what tube…

A: Serial dilution is a method of step-wise dilution of bacterial culture to reduce the concentration…

Q: Joseph, being a know-it-all, told Mary that her method would not include all microbes. To…

A: Joseph and Mary had a disagreement about the effectiveness of Mary's method for counting microbes in…

Q: - What is a pure culture? - Describe the procedure for making a streak plate from a clinical…

A: Introduction A growth media, also known as a culture medium, is a solid, liquid, or semi-solid…

Q: A bacterial culture has a concentration of 3.2 x 108 cells /mL.  You dilute this culture as follows:…

A: CFUs are a measure of the number of progenitors present in a given population of cells; if an…

Q: You perform a serial dilution of a water sample and the 10-7 tube when 0.1 ml was plated onto the…

A: Estimation of the number of viable cells is important in microbiology protocols. It is estimated…

Q: colony-forming units

A: Colony Forming Unit:It is designated as CFU.It can defined as the unit of measurement used to…

Q: List five essential procedures of the aseptic technique when transferring a broth culture to another…

A: The term "aseptic method" refers to a combination of medical practises and procedures that help…

Q: What are the advantages and disadvantages of using the Wet Mount technique?

A: As per bartleby guidelines, we are suppose to answer only one question kindly post other separately.…

Q: This test determines the ability of bacteria to use citrate as source of carbon and inorganic…

A: The citrate test is utilized to check whether a bacterium species can use citrate as a source of…

Q: Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of…

A: Eosin Methylene Blue (EMB) agar is a differential microbiological medium, which somewhat hinders the…

Q: You are given a mix culture of S. aureus,E. coli and P. aeruginosa. Besides the streak plate method…

A: Microbiological research necessitates the use of pure cultures. Spreading bacteria on the surface of…

Q: Mary wanted to know the amount of microbes in her garden. To do this, she took a 10g soil sample,…

A: Serial dilution is a process done to reduce the microbial count in the sample. This is specifically…

Q: What are the materials and methods to achieve identification code (not the actual identification…

A: The bioMerieux catalog/APIweb is a software product that contains all of the API databases for a…

Q: A sample of Earth Alive Soil Activator was diluted by transferring 1 gram into a 9 ml dilution blank…

A: Microorganism concentrations are calculated using serial dilutions. A viable bacterial or fungal…

Q: Describe characteristics of Streptococcus Agalactiae in the Agar: (How does colonies look like…

A: Streptococcus agalactiae is a Gram-positive, non-motile, and facultative anaerobic bacteria. It is…

Q: You are interested in the total bacterial load of the bat guano. In order to determine this, you go…

A: For calculation of total bacterial count we choose the solution that usually gives the colony number…

Q: How would you produce a 10^-1 dilution if a 3 mL bacterial sample using the entire 3 mL volume?…

A: Dilution factor refers to the ratio of the volume of the initial (concentrated) solution to the…

Q: Summarize briefly the pasteurization step. Why is there a need to pasteurize the milk for at least…

A: Note: As Per Guidelines, We Can Answer One Question At A Time. Ask Again To get rest answers.…

Q: but de prod the p ing hacterial clies that appear colrle with ak Salmonello-Shigell (SS) agar plate…

A: Salmonella shigella agar is a selective medium used for identification, isolation, cultivation…