According to the table above, name 1 method that is least effective in killing endospores and explain why.

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According to the table above, name 1 method that is least effective in killing endospores and explain why.
TABLE 9.4 Physical Methods of Microbial Control
Conditions
Method
Moist Heat
Boiling
Autoclaving (pressure
cooking)
Pasteurization
Ultra-high-temperature
sterilization
Dry Heat
Hot air
Incineration
Refrigeration
Freezing
Desiccation (drying)
Lyophilization (freeze-drying)
Filtration
Osmotic Pressure
lonizing Radiation (electron
beams, gamma rays, X rays)
Nonionizing Radiation
(ultraviolet light)
2021 Pearson Education, Inc.
10 min at 100°C (at sea level)
15 min at 121°C
30 minutes at 63°C; 15 sec at 72°C
1-3 sec at 140°C
2 h at 160°C or 1 h at 171°C
1 sec at approximately 1500°C
0-7°C
Below 0°C
Varies with amount of water to be
removed
-196°C for a few minutes while
drying
Filter retains microbes
Exposure to hypertonic solutions
Seconds to hours of exposure
(depending on wavelength of
radiation)
Irradiation with 260-nm-wavelength
radiation
Action
Denatures proteins and destroys
membranes
Denatures proteins and destroys
membranes
Denatures proteins and destroys
membranes
Denatures proteins and destroys
membranes
Denatures proteins, destroys
membranes, oxidizes metabolic
compounds
Oxidizes everything completely
Inhibits metabolism
Inhibits metabolism
Inhibits metabolism
Inhibits metabolism
Physically separates microbes
from air and liquids
Inhibits metabolism
Destroys DNA
Formation of thymine dimers inhib-
Ts DNA transcription and replication
Representative Use(s)
Disinfection of baby Bottles and sanitization of
restaurant.cookware and tableware
Autoclave: sterilization of medical and laboratory
supplies that can tolerate heat and moisture;
pressure cooker: sterilization of canned food
Destruction of all pathogens and most spoilage
microbes in dairy products, fruit juices, beer,
and wine
Sterilization of dairy products
Sterilization of water-sensitive materials, such
as powders, oils, and metals
Sterilization of inoculating loops, flammable
contaminated medical waste, and diseased
carcasses
Preservation of food
Long-term preservation of foods, drugs, and
cultures
Preservation of food
Long-term storage of bacterial cultures
Sterilization of air and of heat-sensitive
ophthalmic and enzymatic solutions, vaccines,
and antibiotics
Preservation of food
Sterilization of medical and laboratory
equipment and preservation of food
Disinfection and sterilization of surfaces and of
transparent fluids and gases
Transcribed Image Text:TABLE 9.4 Physical Methods of Microbial Control Conditions Method Moist Heat Boiling Autoclaving (pressure cooking) Pasteurization Ultra-high-temperature sterilization Dry Heat Hot air Incineration Refrigeration Freezing Desiccation (drying) Lyophilization (freeze-drying) Filtration Osmotic Pressure lonizing Radiation (electron beams, gamma rays, X rays) Nonionizing Radiation (ultraviolet light) 2021 Pearson Education, Inc. 10 min at 100°C (at sea level) 15 min at 121°C 30 minutes at 63°C; 15 sec at 72°C 1-3 sec at 140°C 2 h at 160°C or 1 h at 171°C 1 sec at approximately 1500°C 0-7°C Below 0°C Varies with amount of water to be removed -196°C for a few minutes while drying Filter retains microbes Exposure to hypertonic solutions Seconds to hours of exposure (depending on wavelength of radiation) Irradiation with 260-nm-wavelength radiation Action Denatures proteins and destroys membranes Denatures proteins and destroys membranes Denatures proteins and destroys membranes Denatures proteins and destroys membranes Denatures proteins, destroys membranes, oxidizes metabolic compounds Oxidizes everything completely Inhibits metabolism Inhibits metabolism Inhibits metabolism Inhibits metabolism Physically separates microbes from air and liquids Inhibits metabolism Destroys DNA Formation of thymine dimers inhib- Ts DNA transcription and replication Representative Use(s) Disinfection of baby Bottles and sanitization of restaurant.cookware and tableware Autoclave: sterilization of medical and laboratory supplies that can tolerate heat and moisture; pressure cooker: sterilization of canned food Destruction of all pathogens and most spoilage microbes in dairy products, fruit juices, beer, and wine Sterilization of dairy products Sterilization of water-sensitive materials, such as powders, oils, and metals Sterilization of inoculating loops, flammable contaminated medical waste, and diseased carcasses Preservation of food Long-term preservation of foods, drugs, and cultures Preservation of food Long-term storage of bacterial cultures Sterilization of air and of heat-sensitive ophthalmic and enzymatic solutions, vaccines, and antibiotics Preservation of food Sterilization of medical and laboratory equipment and preservation of food Disinfection and sterilization of surfaces and of transparent fluids and gases
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