Mention and explain at least one reason why you might obtain growth of bacteria on EMB that was supposed to appear green but it resulted in pink color?
Bacterial Morphology
The bacteria are prokaryotic organisms that are single-celled, and are found to exist as free-living and possess a microscopic size. The morphology is found to vary in the bacteria, where some of them are identified as individual organisms and the others are detected as colonies. The size and shape of the bacterial cell also represent its morphology.
Bacterial cell structure
Bacteria are single-celled, tiny creatures that may enter healthy tissues and grow rapidly. Bacteria are microscopic organisms that are tiny and unicellular. These are members of the prokaryote kingdom. They live in water, air, soil, and all-natural environments. They are used in industrial and therapeutic processes, and they support a wide range of plant and animal life. The first organism to appear on the planet. Bacteria-like creatures are the oldest known fossils. Bacteria can consume a wide range of organic and inorganic elements, and some may even survive in harsh conditions.
EMB is selective dye used for isolation of gram negative bacterial ( E.coli and coliforms)
EMB contain eosin Y and methylene blue. the eosin changes colour from dark to purple due to the acidic nature of medium from bacterial growth
EMB also contain lactose and sucrose sugars which help to produce acid from lactose. which play a crucial part in colour changing.
Lactose fermentation also responsible for colour changing.
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