How should the urine sample be taken? And why is this technology chosen? b. A rapid test is performed on the urine sample via a test strip that has reagent fields for nitrite. After 1 min read the strip which is pink colored. Which bacterial species is most likely in the urine sample? c. What is the reservoir for this urinary tract pathogen?
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Young woman applies to her health center with burning and frequent vomiting but has no fever. The doctor diagnoses acute cystitis and the patient is allowed to leave a urine sample.
a. How should the urine sample be taken? And why is this technology chosen?
b. A rapid test is performed on the urine sample via a test strip that has reagent fields for nitrite. After 1 min read the strip which is pink colored. Which bacterial species is most likely in the urine sample?
c. What is the reservoir for this urinary tract pathogen?
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- a. This bacterium is a gram-negative cocci. It can ferment glucose, meaning it is positive for fermentation of glucose test. It can also reduce nitrate. What is it? b. This bacterium is a gram-positive rod. It is negative for the catalase reaction, but does produce gas from glucose. What is it?A 1.5-year-old child developed vomiting, diarrhea, and fever. Stool sample were inoculated into the Endo media. After 18 hours on the surface of the medium grew medium-sized, round, slightly convex red colonies with a metallic luster. The doctor suspected Escherichia coli. 1. Name the composition of the Endo agar media. 2. Describe the properties of bacterial colonies on the Endo media. 3. What purpose differential diagnostic Endo media used for?The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate 1 10 Plate 2 10 Plate 3 10 dilution dilution dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 10 dilution -6 *Too many to count Number of colony forming units (CFU) TMTC* TMTC* 840 28 19 1
- This agar plate was obtained by spreading 0.1 ml of a 1:1,000 dilution of a bacterial sample taken from cole slaw, and then incubating at 37oC for 24h. This plate indicates that: a. there are 42 bacteria/ml on the cole slaw b. there are at least 4 different types of bacteria in the cole slaw c. there are approximately 42,000 bacteria/ml in the cole slaw sample d. there are approximately 420,000 bacteria/ml in the cole slaw e. these bacteria cannot cause illness because they came from the person’s skin who made the cole slawThe number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 0.1 mL aliquot from each dilution was spread onto Plate Count Agar (PCA). The number of bacterial colony forming units (CFU) after overnight incubation are shown as listed in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution Plate 3 107 dilution Plate 4 10 dilution Plate 5 107 dilution Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 382 83 10 2 0Choose the one answer that fits best. Which of the following statements regarding the proper procedure for using Micropipettes is NOT correct? O a. You cannot use a 2-20 µl micropipette to pipet 200 µl O b. To expel all the liquid from the tip, you have to press the eject button O c. To draw up solution, press and hold the plunger at the first stop before entering the solution O d. Micropipettes always require the use of a disposable plastic tip O e. While pipetting, micropipettes should always be held as straight as possible
- You are working in a medical lab and receive a urine sample from a patient that is displaying urinary tract infection symptoms. You use the urine to set up 8 serial 10-fold dilutions. You decide to spread plate 100 µl of different dilutions. The colony count after incubation overnight: 10-4 dilution has 173 colonies. What is the cfu/ml of this culture?Andrew has to prepare 30 plates (use maximum volume), 15 slants (small test tube), and 15 stabs (big test tube) of Luria Bertani Agar. a. What is the total volume of the medium needed? b. What is the amount of each component needed, given the following media composition per liter of distilled water:Jack, a new MLT at Cape Fear Hospital was given the following information from a patient's urine sample which had been tested for the presence of S. aureus. The urine contained 6.4 x 107 CFU/ml S. aureus, the plate dilution was 10, and the sample had been taken from the 10-5 dilution tube. Calculate the number of bacterial colonies which must have been counted on the plate and what volume of the sample in µl that was actually plated to give the above results. PS, both of these values can be calculated.
- The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution Plate 1 10 dilution Plate 2 10 dilution -5 Plate 3 10 dilution Plate 4 10 dilution Plate 5 107 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* TMTC* TMTC* 867 154 18The number of bacterial cells in a culture broth is to be determined by a culture technique. Serial dilutions were performed and a 1 mL aliquot from each dilution was mixed with warm molten agar and poured into a Petri dish. The numbers of bacterial colony forming units (CFU) after overnight incubation are shown in the table below. What is the number of colony forming units per mL of the culture broth? Choose only the most appropriate plate for your calculation. Give your answer as the number only (do not add text for the units). You may use scientific notation with the format 1.12e+6 (that is, 1.12 x 106 cfu/mL). (Note: Canvas will then display your answer a whole number.) Plate Dilution -3 Plate 1 10 dilution Plate 2 10-4 dilution Plate 3 10-5 dilution Plate 4 10 dilution Plate 5 10 dilution -8 Plate 6 100 dilution *Too many to count Number of colony forming units (CFU) TMTC* 1,957 65 21 1 0 mA 7 year old female patient is admitted to a local hospital for a greenstick radius break. The break is repaired, the patient is sent home and receives a cast a few days later. The patient arrives back at the hospital 20 days afterwards complaining of pain, sweeling under the cast. Upon removal of the cast, there is notable swelling, pus, and a furuncle. a. what type of specimen should you collect? b. What type of instant test would you perform? c. if your clinic ran out of the instant tests, what lower cost method would you use to identify the species? d. What would be the typical course of treatment? e. Due to some cases of antibiotic resistant infections at the same hospital, the doctors want to use a specimen and test for antibiotic resistence, What test would you perform, and which antibiotics should the doctor avoid prescribing?