Answered: 1. What could be used to determine if…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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Question

Below is a scenario requiring the use of laboratory equipment and instruments.

A watery fecal sample is tested for the presence of Bacillus cereus and Vibrio cholerae. The sample is stored in a screw capped tube. You are tasked to grow the Bacillus in a solid medium while Vibrio in a liquid medium.

Choices: A. Incubator, B Hanging Drop Slide , C.Petri Dish, D. Drying Oven, E. Bunsen Burner, F. Inoculating NeedlE , G. Volumetric Pipette, H. Screw-capped Tubes, I. Hot plate, J. Centrifuge, K. Inoculating Loop, L. Microscope, M. Beaker, N. Biosafety Cabinet

1. What could be used to determine if there are more bacteria in the sample aside from Bacillus and Vibrio?

2. What should you use if you want to observe the natural motility of Bacillus under a Brightfield microscope?

3. Where should you perform the task of sterilization and inoculation? 4.What should be used to transfer the sample to the broth?

5. What could be used to separate the solid from the liquid portion of the fecal sample?

6.Vibrio resulted to a pinkish color under gram staining. What instrument was used to heat fix the microbe?

7. Where should you store the sample if you want to overgrow Vibrio?

8.What do you use to dry and sterilize all the glass wares simultaneously?

9.What is the best equipment to heat the solid and liquid media within a regulated temperature?

10. If an agar slant is required for Bacillus, what glassware should be used?