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What Gram-negative bacterium might you come into contact with in a unsanitary hot
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- If a yellow halo is present around a colony on a mannitol salt agar (MSA) plate, the bacterium cannot ferment mannitol. true or falseBelow is an MSA plate inoculated with bacteria and grown at 37*C for 2 days. Be pecific and thorough in your answers for parts a and b below. The color of the plate is yellow because b) The presence of bacterial colonies indicates that the bacteria can surviveWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tube
- What will pose an ethical issue in these procedures are performed - sterilization?Purpose Solve the identity of an unknown bacterial specimen by creating a dichotomous key and using the staining, culturing and biochemical identification procedures you have learned about during the semester. Possible Organisms Alcaligenes faecalis Enterobacter aerogenes Enterococcus faecalis Escherichia coli Proteus vulgaris Pseudomonas aeruginosa Salmonella arizoniae Staphylococcus aureus Staphylococcus epidermidis Staphylococcus saprophyticus Streptococcusbovis Streptococcus pyogenes You must write up your OWN dichotomous key for all the possible unknown organisms listed on above. Writing this key requires you use the same type of reasoning used in the Dichotomous key lab. The first step of the key will be the Gram Stain. Subsequent steps will include biochemical tests only. Please help me with this question. Thank you so much !Give the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinet 11. Bacteriologic filters (Seitz, Chamberlain, Berkfield) 12. Petri dish 13. Culture tubes 14. Hanging drop slide 15. Durham’s tube 16. Staining rack 17. Thermostatically controlled water bath 18. Inoculating loop 19. Inoculating needle 20. Vials
- Below are photos of 3 bacteria, letter lableled, inoculated onto MSA plates and incubated. Note no growth on plate C MSA (Mannitol Salt Agar) Match the descriptions below to the correct letter labeled Bacteria This bacterium cannot grow in high saltGive the uses/functions and images of each apparatuses. Basic Laboratory Equipment Uses/Functions Picture 1. Microscope 2. Colony Counter 3. Autoclave 4. Microbiology incubator 5. Drying oven 6. Refrigerator (microbiology) 7. Bunsen burner/alcohol lamp 8. Candle jar 9. Anaerobic jar 10. Microhood or Bacteriologic hood/Safety hood/Safety cabinetGeneral Instructions:Choose 1 bacteria/diseases and fill out the details. An example of an answered template is also provided for your reference. Causative Agent and Disease Profile for S. aureus Template and Example ITEM MSM PROFILE MICROBIAL PROFILE I MICROORGANISM/CAUSATIVE AGENT Staphylococcus aureus A GRAM REACTION (+) B OXYGEN REQUIREMENT Facultative Aerobes C SIZE 1.5 µm D SHAPE Cocci in clusters E HABITAT Normal flora of skin/anterior nares/pharynx F DISCOVERY G MICROSCOPIC IMAGE II DISEASE PROFILE Scalded skin syndrome A DISEASE/S Skin and Wound Infections Scalded Skin Syndrome Toxic Shock Syndrome Food Poisoning Pneumonia B SYMPTOMS OF THE DISEASE A high fever · Nausea and vomiting · A rash on your palms and soles that resembles a sunburn C INCUBATION PERIOD 2 and 4 hours (range 30 minutes to 8 hours) D MODE OF…
- Match the description to the correct medium This medium is enrichment for fastidious [Choose ] bacterial pathogens and differential based on hemolysis [Choose ] This medium is selective based on Tryptic Soy Agar, TSA salt/sodium chloride tolerance at 7.5 % Sabouraud Dextrose Agar, "Sab" agar weight/volume and differential based on mannitol fermentation Mannitol Salt Agar, "MSA" MacConkey Agar, "Mac" agar This medium is selective based on the ability to grow in bile salts and crystal violet and is EMB Eosin Methylene Blue Agar differetial based on lactose fermentation Blood Agar, "BA" this medium is all purpose and can grow a [Choose ] wide range of bacteria and fungiGreetings :) Based on the results of using Hanging-drop method in Figure 2, what microorganisms can you find? Kindly state or identify all microbes you can see, and briefly described each of them. Thank you so much!What is the physical appearance (solid, liquid, semi-solid) of following media: agar plate