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1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach File

1 ml from main culture 1ml 99999 5 ml LB 1 ml 5 ml LB 5ml LB 1ml 5 ml LB 1 ml 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach File

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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1 ml from main culture 5 ml LB 1 ml 1ml 1ml 5 ml LB I ? 5ml LB 1 ml 5 ml LB 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach File
Transcribed Image Text:1 ml from main culture 5 ml LB 1 ml 1ml 1ml 5 ml LB I ? 5ml LB 1 ml 5 ml LB 5 ml LB You took 250 µl of the last dilution and planted it on solid medium. 50 colonies were counted A total of 10 ml of microbial culture (liquid LB+ e.coli) was brought to your laboratory in a container. This sample contains the bacteria Escherichia coli. The person who brought in wants to know how many colonies (cfu) there are in the culture. You have started the experiment by taking 1 ml from the culture and then added into the first tube which contain 5 ml of LB. After that, you diluted the sample with a dilution rate of 1/6 with 4 times (take 1 ml in 5 ml of LB medium (so total 6 ml)). You took 250 μl of the last dilution and planted it on solid medium and when counted after incubation, 50 colonies were counted. How many cfu/ ml bacteria are there in the sample (main stock) in total? (please show all your calculations and steps). Attach File
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