4 Which of the following graphs, A or B, illustrates the typical behavior of a solid dissolving in water? Explain your choice. A 100 90 B 100 Solubility (g solute in 100 mL H₂O) 80 70 60 50 40 30 20 10 0 0 10 KCI Solubility (g solute in 100 mL H2O) 90 80 70 60 50 Na₂SO4 40 30 20 10 0 20 20 30 40 50 60 70 80 90 100 0 10 20 30 40 50 60 70 Temperature (°C) Temperature (°C) 5 Why is it important to measure the masses of the NaCl and KNO, samples in Part B? - 80 90 100
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- A water solution contains 60% Na2S202 together with 1% soluble impurity. Upon cooling to 10°C, Na2S202 • 5 H2O crystallizes out. The solubility of this hydrate is 1.4lb Na2S202 • 5 H20/lb free water. The crystals removed carry asadhering solution 0.06 lb solution/lb crystals. When dried to remove the remaining water (but not the water of hydration), the final dry Na2S202 5 H20 crystals must not contain more than 0.1% impurity. To meet this specification, the original solution, before cooling, is further diluted with water. On the basis of 100 Ib of the original solution,calculate (a) the amount of water added before cooling, and (b) the percentage recoveryof the Na2S202 in the dried hydrated crystalsYou need to make a protein buffer of: . • • 100 mM NaCl 25 mM Tris 8 5% w/v glycerol • 2 mM DTT Your stock solutions are: 5 M NaCl • 2 M Tris 8 • 70% w/v glycerol • DTT @ 154.25 g/mol How would you make a 1L protein buffer Solution? Show your work and describe.Table 2. Volume of BSA, protein content, and absorbance readings of reference solutions Solution Volume of BSA standard solution (μL) Protein content(μg/mL) Absorbance value At 595 nm 1 0 0 0 2 10 1 0.022 3 30 3 0.065 4 50 5 0.106 5 70 7 0.178 6 100 10 0.299 7 120 12 0.380 Make a graph by plotting the absorbance values versus the BSA protein content (in μg) for theseven reference solutions. When constructing the graph, be…
- When 2.43 g of a nonelectrolyte solute is dissolved in water to make 435 mL of solution at 25 °C, the solution exerts an osmotic pressure of 895 torr. What is the molar concentration of the solution? concentration: 0.045 M Incorrect How many moles of solute are in the solution? moles of solute: 0.034 mol Incorrect What is the molar mass of the solute? molar mass: 69.12 g/mol Incorrect3 mL of a 45 mM stock solution of a substrate is added to 8 mL of water. Calculate the following values. The substrate molecular weight is 125 g/mol. Calculate the following values: Substrate Volume in mL Dilution Factor Substrate number of moles Substrate concentration for the diluted solution in mM Substrate concentration for the diluted solution in mole/L Substrate concentration for the diluted solution in mg/mLConsider a mixture comprised of the proteins attached: Protein that will most strongly bind to an anion exchange column? Protein that will elute first in gel filtration chromatography? Protein that will elute last in hydrophobic interaction chromatography? Protein that will elute last in carbohydrate containing column?
- Size Size Crude Anion Cation 2) Exclusion Exclusion Lysate Exchange Exchange 1 2 Total Protein Concentration 15.2 6.6 2 3.75 4.7 (mg/mL) Final Sample Volume 60 30 20 4 3 (mL) Enzyme Specific Activity 0.43 1.7 7.25 18.3 12.5 (units/mgprotein) Based on your protein purification sample data, in which of the purification steps in your protocol did you effectively purify your enzyme? Select all that apply. a) anion exchange correct b) cation exchange correct c) size exclusion 1 correct d) size exclusion 2100 90 NaNO 80 70 Pb(NO,)2 KNO3 60 СаCl 50 40 NaCI KCI 30 20 KCIO3 10 Ce,(SO,)3 10 20 30 40 50 60 70 80 90 100 Temperature (°C) When 50 grams of KCI is dissolved in 100 grams of water at 50 °C, the solution can be correctly described as: saturated supersaturated unsaturated Solubility (g of salt in 100 g H,O) K,Cr,O,I want to make a buffer solution containing 1 M glycene pH = 10 (Na+), 1 mM ZnCl2 , 1 mM MgCl2. I have the stock solutions 1 M glycene, 1 M MgCl2, 1 M ZnCl2, and 10 M NaOH. My final volume of buffer solution needs to be 250ml. how much (in ml) of each solution should i put into my buffer?
- How much of the enzyme proteinase k (solute) is required to make 250ml of a solution with a concentration of .01mg/ml? (Weight/volume ratio)1. How would you make 10 L of 0.3 M citrate buffer pH 2.5 from solutions of 0.4 M NaCitrate and 0.4 M citric acid? Include the amounts of all components added in mL. pKa = 3.09 2. How many grams of disodium hydrogen phosphate (142 g/mol), mL of 0.5 M HCl or NaOH, and mL of water are needed to make 500 mL of 0.15 M phosphate buffer pH 11.90? What would be the pH of this solution if it were diluted to 2 L with pure water? pKa(1) = 2.15; pKa(2) = 6.86; pKa(3) = 12.38 3. How many mL of 3 M NaOH or 3 M HCl and mL of 0.5 M acetate buffer pH 3.78 do you need to make 600 mL of a 0.02 M acetate buffer pH 5.3?Acetic acid is the principal ingredient in vinegar as shown; that's why it tastes sour. At equilibrium, a solution contains [CH3CO2H] = 0.0787 M and [H3 O+] = [CH3 CO2−] = 0.00118 M. What is the value of Ka for acetic acid?