3. The Keq for imidazole binding to hemoglobin can be determined from the following linear equation, derived from measured lab data. It is used mainly because we will never be directly measuring A100, as it is a theoretical limit that cannot be reached. 1. 1. 1. ДА ()- ba 001v [u] If the reciprocal of the change in absorbance (1/AA) is plotted versus the reciprocal of the imidazole concentration (1/[Im]), the slope and the y-intercept are important in interpreting the result. Clearly mark a square around the term equivalent to slope and a triangle around the y-intercept. a. b. If the best-fit line for your data gave a slope of 0.208 M and an intercept of 21.3, determine values of AA100 and Keg? Answer in this box: = 001 = b Using the calculated Keg in part b of this question and an [Im] = 0.114 M, what ratio of bounc to unbound ferrihemoglobin would C. you predict? Answer in this hox:

3. The Keq for imidazole binding to hemoglobin can be determined from the following linear equation, derived from measured lab data. It is used mainly because we will never be directly measuring A100, as it is a theoretical limit that cannot be reached. 1. 1. 1. ДА ()- ba 001v [u] If the reciprocal of the change in absorbance (1/AA) is plotted versus the reciprocal of the imidazole concentration (1/[Im]), the slope and the y-intercept are important in interpreting the result. Clearly mark a square around the term equivalent to slope and a triangle around the y-intercept. a. b. If the best-fit line for your data gave a slope of 0.208 M and an intercept of 21.3, determine values of AA100 and Keg? Answer in this box: = 001 = b Using the calculated Keg in part b of this question and an [Im] = 0.114 M, what ratio of bounc to unbound ferrihemoglobin would C. you predict? Answer in this hox:

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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SPECTROPHOTOMETRY Two groups in a BIO 120 lab extracted a blue-violet pigment from mayana plant which was to be used as a natural dye for Philippine textiles. To determine the concentration of the pigment isolated, each group made a standard curve using commercially available indigo dye in dimethylsulfoxide (DMSO). The following data were obtained by the two groups: Table 4. Absorbance readings of standard solutions of varying concentrations of indigo dye in DMSO. Pure DMSO 0.115 Standard solutions (ppm) Group 1 Data Group 2 Data 0.2 0.398 0.224 0.4 0.563 0.437 0.6 0.732 0.603 0.8 0.833 0.811 1.0 1.008 0.966 1. Show the constructed standard curves of the two groups with proper figure titles. 2. Which of the two groups generated a better standard curve? Justify your answer by providing the R? value for each data set. 3. The extracted pigment has an absorbance of 0.983. When a 0.2 mL-aliquot of the extract was diluted to 1.0 mL, the absorbance of the diluted solution was found to be…
There is a substance in a solution (8 g/liter). The length of cuvette is 2 cm and the extinction coefficient is 0.0376 M-1 cm-1, how much is the beam of light is transmitted?
Iron(III) forms a complex with thiocyanate ion that has the formula Fe(SCN)+2. The complex has an absorption maximum at 580nm. A sample of well water was assayed according to the following scheme: Sample Sample Vol (mL) Oxidizer Fe(ii) (mL) added 2.75ppm KSCN (0.050 M) H20 (mL) Abs @ 580 nm 1 50.00 5.00 5.00 20.00 20.00 .549 2 50.00 5.00 0 20.00 25.00 .231 What is the concentration of Fe in the well water sample?
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A student prepared an equilibrium system using Fe(NO3)3 and KSCN, which reacted to produce iron(III) thiocyanate by the balanced reaction shown below. Fe3+ + SCN' + FESCN2+ Previously the student prepared a Beer's Law calibration plot of absorbance (y-axis) versus FESCN2+ (x-axis) and calculated a trendline with the equation of y = 3229x. The student then prepared another equilibrium mixture using the following amounts. 0.00200 M Fe(NOa)a (mL) 0.00200 M KSCN (mL) 0.1 M HNO3 (mL) Absorbance 6.00 3.00 16.00 0.258 Use the above data to find initial and equilibrium concentrations of each species and use an ICE table to calculate Keg. [ Fe+ ] initial: [ SCN' ] initial: [ FESCN2+ ] initial: [Fe3+ ] at equilibrium: [ SCN' ] at equilibrium: [ FESCN2+ ] at equilibrium: Keg =