Tryptophan absorbs at a maximum wavelength of 280 nm. In a cuvette (1 cm-optical path length), a 200 µM tryptophan solution shows an absorbance value at 280 nm of 1.2. 1- Calculate & the molar absorption coefficient (also called molar extinction coefficient) of tryptophan. A solution of protein X, at the concentration of 25 µM, shows an absorbance value at 280 nm of 0.6. The protein X has no tyrosine and no phenylalanine residues. 2- Determine the number of tryptophan residues of the protein X.

Tryptophan absorbs at a maximum wavelength of 280 nm. In a cuvette (1 cm-optical path length), a 200 µM tryptophan solution shows an absorbance value at 280 nm of 1.2. 1- Calculate & the molar absorption coefficient (also called molar extinction coefficient) of tryptophan. A solution of protein X, at the concentration of 25 µM, shows an absorbance value at 280 nm of 0.6. The protein X has no tyrosine and no phenylalanine residues. 2- Determine the number of tryptophan residues of the protein X.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

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