Tryptophan absorbs at a maximum wavelength of 280 nm. In a cuvette (1 cm-optical path length), a 200 µM tryptophan solution shows an absorbance value at 280 nm of 1.2. 1- Calculate & the molar absorption coefficient (also called molar extinction coefficient) of tryptophan. A solution of protein X, at the concentration of 25 µM, shows an absorbance value at 280 nm of 0.6. The protein X has no tyrosine and no phenylalanine residues. 2- Determine the number of tryptophan residues of the protein X.

Tryptophan absorbs at a maximum wavelength of 280 nm. In a cuvette (1 cm-optical path length), a 200 µM tryptophan solution shows an absorbance value at 280 nm of 1.2. 1- Calculate & the molar absorption coefficient (also called molar extinction coefficient) of tryptophan. A solution of protein X, at the concentration of 25 µM, shows an absorbance value at 280 nm of 0.6. The protein X has no tyrosine and no phenylalanine residues. 2- Determine the number of tryptophan residues of the protein X.

Chemistry

10th Edition

ISBN:9781305957404

Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste

Chapter1: Chemical Foundations

Section: Chapter Questions

Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...

See similar textbooks

Similar questions

Assuming that the sample optical path length is 1 cm, find the molar absorptivity at wavelength 1 for MR- in cm-1 M-1 given the following: Absorbance at wavelength 1 = 0.322 concentration of MR= = 0.157 M concentration of HMR = 0.146 M molar absorptivity of HMR at wavelength 1 = 0.417 cm-1 M-1
You have a 2.5 M aqueous sample of a metal ion, which has an absorbance of 0.75 at 660 nm. An unknown solution of the same metal ion, on the same day Review Beer's Law. and on the same spectrophotometer, has a measured absorbance of 0.59. What is the concentration of metal ion in the unknown solution? In vour notohookı
Q/ If the intensity of the transmitted ray is equal to 0.12 of the intensity of the incident ray on a solution in a cell thickness of 2 cm and the molar absorption coefficient is 0.35 L.Mol-1.cm-1, calculate the absorbance and concentration of the solution
An absorption measurement with a 1 cm path length yields a reading of 0.004 absorbance units with a noise of 0.001 absorbance units and a mean noise reading of 0 for 10 scans averaged together. Assuming the noise for a single scan does not change if the path length is increased to 6 cm and the number of scans is increase to 100, what is the signal to noise of the new measurement.
The max of aspirin (molar mass 180g/mol) in neutral solution is 225 nm and & is 650 mol-¹Lcm ¹. An absorbance of 1.80 was measured for a solution of aspirin, with a 1 cm path length. Calculate the concentration of the aspirin solution.
"[FeSCN2+]eq is calculated using the formula: [FeSCN2+]eq = Aeq/Astd x [FeSCN2+]std where Aeq and Astd are the absorbance values for the equilibrium and standard sample respectively." I am confused about what Aeq and Astd mean. We did an experiment where we measured the absorption of this chemical formation and I got around 0.18. Is that one of the values?
a) If an unknown sample containing FeSCN2+ has an absorbance of 0.542, what is the concentration of FeSCN2+ in the unknown sample? b) What are the units of concentration? c) If the path length of light is 13.5 mm (millimeters), what is the absorptivity coefficient, ?ε, for FeSCN2+? d) What are the units of the absorptivity coefficient, ?ε?
PLEASE ANSWER ASAP
Calculate the concentration of a colored solution if the molar absorptivity is 39.80 M-1•cm-1 and the absorbance is 0.777. The path length of the sample is 1.00 cm.
Please don't provide handwritten solution ....
The Beer’s Law plot of the standard curve for the equilibrium experiment was determined by plotting? absorbance versus [Fe3+] absorbance versus % transmittance % transmittance versus [FeSCN2+] absorbance versus [FeSCN2+]
What is the molar extinction coefficient at 625 nm for this copper (II) acetate solution? How was it determined? Standard Solution Concentration (M) Absorbance 1 0.025 0.049 2 0.050 0.153 3 0.100 0.242 4 0.200 0.499 5 0.250 0.694