Assay # 1 2 3 4 PNPP, conc. μΜ 150 75 37.5 18.75 Volume of 4 mM PNPP, mL 0.15 0.075 0.0375 0.01875 Volume of 0.2 M Tris pH 8, mL 0.4 0.4 0.4 0.4 Volume of H₂O, mL 3.35 3.43 3.46 3.48 Volume of 1/4 enzyme solution, mL 0.100 0.100 0.100 0.100 Total volume, mL 4 4 4 4
Assay # 1 2 3 4 PNPP, conc. μΜ 150 75 37.5 18.75 Volume of 4 mM PNPP, mL 0.15 0.075 0.0375 0.01875 Volume of 0.2 M Tris pH 8, mL 0.4 0.4 0.4 0.4 Volume of H₂O, mL 3.35 3.43 3.46 3.48 Volume of 1/4 enzyme solution, mL 0.100 0.100 0.100 0.100 Total volume, mL 4 4 4 4
Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
Related questions
Question
What is the extinction coefficient for the following absorbances?
1. 3.000
2. 2.022
3.1.2025
4. 0.824
Would the units be in µmoles/mL)^-1 cm^-1
![Assay #
1
2
3
4
PNPP, conc.
μΜ
150
75
37.5
18.75
Volume of
4 mM
PNPP,
mL
0.15
0.075
0.0375
0.01875
Volume of 0.2
M Tris pH 8,
mL
0.4
0.4
0.4
0.4
Volume of
H₂O, mL
3.35
3.43
3.46
3.48
Volume of 1/4
enzyme solution,
mL
0.100
0.100
0.100
0.100
Total volume,
mL
4
4
4
4](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2Fd4f3ff3b-fb46-4808-a023-d60dd0915f27%2F7d6ed801-9941-4ac9-b33d-04180f959085%2Fgjhm8ye_processed.jpeg&w=3840&q=75)
Transcribed Image Text:Assay #
1
2
3
4
PNPP, conc.
μΜ
150
75
37.5
18.75
Volume of
4 mM
PNPP,
mL
0.15
0.075
0.0375
0.01875
Volume of 0.2
M Tris pH 8,
mL
0.4
0.4
0.4
0.4
Volume of
H₂O, mL
3.35
3.43
3.46
3.48
Volume of 1/4
enzyme solution,
mL
0.100
0.100
0.100
0.100
Total volume,
mL
4
4
4
4
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