Assay # 1 2 3 4 PNPP, conc. μΜ 150 75 37.5 18.75 Volume of 4 mM PNPP, mL 0.15 0.075 0.0375 0.01875 Volume of 0.2 M Tris pH 8, mL 0.4 0.4 0.4 0.4 Volume of H₂O, mL 3.35 3.43 3.46 3.48 Volume of 1/4 enzyme solution, mL 0.100 0.100 0.100 0.100 Total volume, mL 4 4 4 4

Chemistry
10th Edition
ISBN:9781305957404
Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Publisher:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCoste
Chapter1: Chemical Foundations
Section: Chapter Questions
Problem 1RQ: Define and explain the differences between the following terms. a. law and theory b. theory and...
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Question

What is the extinction coefficient for the following absorbances? 

1. 3.000

2. 2.022

3.1.2025

4. 0.824

Would the units be in µmoles/mL)^-1 cm^-1 

Assay #
1
2
3
4
PNPP, conc.
μΜ
150
75
37.5
18.75
Volume of
4 mM
PNPP,
mL
0.15
0.075
0.0375
0.01875
Volume of 0.2
M Tris pH 8,
mL
0.4
0.4
0.4
0.4
Volume of
H₂O, mL
3.35
3.43
3.46
3.48
Volume of 1/4
enzyme solution,
mL
0.100
0.100
0.100
0.100
Total volume,
mL
4
4
4
4
Transcribed Image Text:Assay # 1 2 3 4 PNPP, conc. μΜ 150 75 37.5 18.75 Volume of 4 mM PNPP, mL 0.15 0.075 0.0375 0.01875 Volume of 0.2 M Tris pH 8, mL 0.4 0.4 0.4 0.4 Volume of H₂O, mL 3.35 3.43 3.46 3.48 Volume of 1/4 enzyme solution, mL 0.100 0.100 0.100 0.100 Total volume, mL 4 4 4 4
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