3. Prostaglandins (5) are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid () to PGG2 (5 G2), the immediate precursor of many different prostaglandins. a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme (note, ¤¯↑¤µ 的单位) [Arachidonic Rate of formation acid] of PGG₂ (MM) (mm/min) 0.5 1.0 1.5 2.5 3.5 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG₂ with 10 mg/ml ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 b) Ibuprofen () is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. You have learned the three mechanisms() for enzyme inhibition, what they are and how Vmax and Km changed? (you can illustrate as a table) c) Using the data in the first and third columns of the table, calculate the Vmax and Km of the enzyme in ibuprofen inhibition and determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase (note: The calculated numbers varied less than 5% were consider as no change).
3. Prostaglandins (5) are derived from the 20-carbon fatty acid arachidonic acid in a reaction catalyzed by the enzyme prostaglandin endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to convert arachidonic acid () to PGG2 (5 G2), the immediate precursor of many different prostaglandins. a) The kinetic data given below are for the reaction catalyzed by prostaglandin endoperoxide synthase. Focusing here on the first two columns, determine the Vmax and Km of the enzyme (note, ¤¯↑¤µ 的单位) [Arachidonic Rate of formation acid] of PGG₂ (MM) (mm/min) 0.5 1.0 1.5 2.5 3.5 23.5 32.2 36.9 41.8 44.0 Rate of formation of PGG₂ with 10 mg/ml ibuprofen (mm/min) 16.67 25.25 30.49 37.04 38.91 b) Ibuprofen () is an inhibitor of prostaglandin endoperoxide synthase. By inhibiting the synthesis of prostaglandins, ibuprofen reduces inflammation and pain. You have learned the three mechanisms() for enzyme inhibition, what they are and how Vmax and Km changed? (you can illustrate as a table) c) Using the data in the first and third columns of the table, calculate the Vmax and Km of the enzyme in ibuprofen inhibition and determine the type of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase (note: The calculated numbers varied less than 5% were consider as no change).
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
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Question
![3. Prostaglandins (5) are derived from the 20-carbon fatty acid
arachidonic acid in a reaction catalyzed by the enzyme prostaglandin
endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to
convert arachidonic acid () to PGG2 (G2), the
immediate precursor of many different prostaglandins.
a) The kinetic data given below are for the reaction catalyzed by
prostaglandin endoperoxide synthase. Focusing here on the first two
columns, determine the Vmax and Km of the enzyme (note,
¤¯↑¤Ð
的单位)
[Arachidonic Rate of formation
acid]
of PGG₂
(MM)
(mm/min)
0.5
1.0
1.5
2.5
3.5
23.5
32.2
36.9
41.8
44.0
Rate of formation
of PGG₂ with 10
mg/mL ibuprofen
(mm/min)
16.67
25.25
30.49
37.04
38.91
b) Ibuprofen () is an
inhibitor of prostaglandin endoperoxide synthase. By inhibiting the
synthesis of prostaglandins, ibuprofen reduces inflammation and pain. You
have learned the three mechanisms() for enzyme inhibition, what they
are and how Vmax and Km changed? (you can illustrate as a table)
c) Using the data in the first and third columns of the table, calculate the
Vmax and Km of the enzyme in ibuprofen inhibition and determine the type
of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase
(note: The calculated numbers varied less than 5% were consider as no
change).](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F23db8996-a9da-479d-afbe-469ef2e27772%2Fb07c7c86-08e3-43ef-a81e-fa99c7f2ee80%2Fo33yh9l_processed.png&w=3840&q=75)
Transcribed Image Text:3. Prostaglandins (5) are derived from the 20-carbon fatty acid
arachidonic acid in a reaction catalyzed by the enzyme prostaglandin
endoperoxide synthase. This enzyme, a cyclooxygenase, uses oxygen to
convert arachidonic acid () to PGG2 (G2), the
immediate precursor of many different prostaglandins.
a) The kinetic data given below are for the reaction catalyzed by
prostaglandin endoperoxide synthase. Focusing here on the first two
columns, determine the Vmax and Km of the enzyme (note,
¤¯↑¤Ð
的单位)
[Arachidonic Rate of formation
acid]
of PGG₂
(MM)
(mm/min)
0.5
1.0
1.5
2.5
3.5
23.5
32.2
36.9
41.8
44.0
Rate of formation
of PGG₂ with 10
mg/mL ibuprofen
(mm/min)
16.67
25.25
30.49
37.04
38.91
b) Ibuprofen () is an
inhibitor of prostaglandin endoperoxide synthase. By inhibiting the
synthesis of prostaglandins, ibuprofen reduces inflammation and pain. You
have learned the three mechanisms() for enzyme inhibition, what they
are and how Vmax and Km changed? (you can illustrate as a table)
c) Using the data in the first and third columns of the table, calculate the
Vmax and Km of the enzyme in ibuprofen inhibition and determine the type
of inhibition that ibuprofen exerts on prostaglandin endoperoxide synthase
(note: The calculated numbers varied less than 5% were consider as no
change).
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