3.) A mixture of amino acids was subjected to paper chromatography with six amino acidstandards in order to determine its composition. After visualization using ninhydrin, themeasured distances were as follows:ABсDEFGa.) What is the Rf value of each amino acid observed?XDistances:Solvent 10.3cmA4.50cmB - 1.32cmC - 6.60cmD - 5.20cmE - 3.52cmF8.70cmG - 10.26cmX1 - 9.72cmX2 - 4.3cmX3 7.1cm-b.) What are the possible components of the amino acid mixture? Answer can be up to twoamino acids per component.

Paper chromatography is an in expensive powerful analytical technique to separate the solute…

Answered: 3.) A mixture of amino acids was…

Curren'S Math For Meds: Dosages & Sol

11th Edition

ISBN:9781305143531

Author:CURREN

Publisher:CURREN

Chapter9: Parenteral Medication Labels And Dosage Calculation

Section: Chapter Questions

Problem 6.7P

See similar textbooks

Similar questions

Four known amino acids (AA) produce a chromatograph with Rf values as follows: AA1 =0.25, AA2 =0.66, AA3=0.50,AA4=0.80. A mixture of three amino acids yields spots that are 2 cm,5.2cm, and 6.4 cm from the organ. The solvent front is 8.0 cm from the origin. Which three of the four amino acids are contained in the mixture?
2.) An unknown amino acid was subjected to paper chromatography with five amino acid standards in order to identify it. After visualization using ninhydrin, the distances travelled were measured as follows: A B с D E X Distances: Solvent - 9.3cm A - 1.33cm B - 4.75cm C - 2.44cm D - 7.62cm E - 8.75cm X - 1.45cm a.) What is the Rf value of each amino acid sample? b.) Arrange the amino acids from most non-polar to least non-polar (include sample X). c.) What is the possible identity of the unknown amino acid? Answer can be up to two amino acids.
The calibration curve shown below was used to analyze an unknown protein solution. What is the concentration of the unknown solution, if the absorbance of the unknown is 0.505? Answer in ug/mL. 1.2 y%3D0.005x+0.061 R=0.992 0.8 0.6 0.4 0.2 50 100 150 200 250 Concentration ug/ml Absorbance
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography.The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
An amino acid mixture of phenylalanine, glycine and glutamic acid is to be separated by paper chromatography. The solvent is less polar than water. Which of these amino acids will have the highest Rf value and which the lowest? Explain.
You assay 0.5 ml of 1/2000 diluted egg white for protein, as described in the practical schedule, and the spectrophotometer reading at 595 nm is 0.6. Using the attached calibration curve(click to enlarge), calculate the concentration of protein in undiluted egg white. Give your answer in mg/ml.
One tenth mL of a protein solution was diluted with 2.5 mL of water. The A280 of the diluted solution was 0.25. How many mL of the original protein solution and water should be mixed to make 1.0 mL of solution of A280 = 0.80?
1) A solution is made by combining 128 mL of ethanol and 0.452 L of water. In this solution, what is the solvent? What is the solute?2) If a solution contains 128 mL of ethanol and water to make 0.452 L of solution, what is the (v/v)% concentration?3) How many grams of sodium carbonate are needed to make 350 mL of a 0.5% (w/v) sodium carbonate solution?4) What is the molarity of a magnesium chloride solution made by dissolving 8.7g of magnesium chloride in 2.45 L of water?5) An exercise in lab requires you to make 500 mL of a 2.3M beryllium sulfide solution. How many moles of beryllium sulfide will you need?6) How many grams of sodium chloride are needed to make 0.60L of a 2.3M sodium chloride solution?7) How hot must the air in a balloon be heated if initially it has a volume of .750 L at 20°C and the final volume be 1.00 L?8) Convert 455 mmHg to atm.9) A container of gas has an initial pressure of 0.8 atm. The pressure is increased to 1.1atm and the final volume of gas is 2.3 liters.…
A mixture of lipids containing phosphatidic acid, cholesterol, testosterone, and phosphatidylcholine was applied to a hydrophobic interaction chromatography column. The column was then washed with increasingly nonpolar solvents. IWhat order would the lipids be eluted from the column?
An ion-exchange chromatographic separation is performed using a diethyl-aminoethyl- (DEAE)-sepharose column to separate proteins in a mixture. The mixture contains Protein A (pl=6.0), B (pl35.0), C (pl=7.5), D (pl =1), and E (pl=4.0). The protein mixture is prepared in a buffer solution pH =5. When the protein mixture is loaded onto the column, and the column is washed with a buffer solution pH 5, which protein(s) will be captured by DEAE-sepharose in the column? O Protein B because it is predominantly in net negative charge form. O Proteins D and E because they have predominately net negative charge in pH 5 solution O Proteins A, C, D and E because they have charges O Proteins A and C because both both predominantly have net positive charges O Proteins Band E because both predominantly have net positive charges
You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?
The pH probe/meter uses following equations: Ecell = L + 0.0592 log a1 = L - 0.0592 pH Where L = L1 + EAg/AgCI + Easy= constants L1 = - 0.0592 log a2 a1 = activity of analyte solution a2 = activity of internal solution How will measured pH value be affected vs “real” pH if the temperature of the sample is 30C when pH was measured ? a.measured pH is lower than real pH b.impact can not be determined c.measured pH is higher than real pH d.measured pH is same as real pH

SEE MORE QUESTIONS

Recommended textbooks for you

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

Curren'S Math For Meds: Dosages & Sol

Nursing

ISBN:

9781305143531

Author:

CURREN

Publisher:

Cengage

SEE MORE TEXTBOOKS

3.) A mixture of amino acids was subjected to paper chromatography with six amino acid standards in order to determine its composition. After visualization using ninhydrin, the measured distances were as follows: