You have a 20 mg/mL stock solution of the amino acid arginine and need to make plates containing arginine at a concentration of 10 micrograms/mL. How much of the stock solution do you add to 1 liter of plate media?

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Q: You want to set up a 1:10 dilution series, so that you can plate a 10-1, 10-2, and a 10-3dilution;…

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Q: How do you prepare 50 ml of 20 mg/ml proteinase K from 50 mg/ml stock solution

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Q: Gel used in electrophoresis is/are: a. Agarose gel b. Polyacrylamide plain gel c. Polyacrylamide SDS…

A: Electrophoresis is a method used to separate mixtures of RNA , DNA or proteins according to their…

Q: Three dipeptides are separated by electrophoresis at ph 5.8. if the dipeptides are Arg-trp, Asp-thr,…

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Q: How is it that smaller molecules move through a gel-filtration column more slowly than larger…

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Q: How do you calculate the amount of ammonium sulfate necessary to get a 20% solution?

A: Ammonium sulfate is the most commonly used for protein precipitation in protein purification.

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Q: 4 mL of 10% TCA solution was added to 1 mL of serum and after mixing, it was waited for two minutes…

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Q: If you have to prepare 4 nM solution of human IgG ( immunoglobuline) which has molecular mass of 150…

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Q: n SDS PAGE the resolving gel :   is 4%(w/v) acrylamide,PH )6.8   is 6-20% (w/v) acrylamide…

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Q: the following questions about protein concentration determination. a) At what wavelength should an…

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Q: Using ultrafiltration, you concentrate a solution of Protein X as follows: Initial volume protein X…

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Q: How should you prepare your running buffer?

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Q: Each liter of an intranasal antibiotic solution contains 160 mg of gentamicin. You have 2-mL…

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Q: what is the dna concentration suspended in PO4 buffer at ph 7.0 that has an absorbance at 260 nm…

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Q: You are using a spectrophotometer to see whether you accurately performed a serial dilution of a…

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Q: Figure 3: ● • . ● KDa ● 97.4 66.2 45.0 ● 31.0 21.5 14.4 S-1 p-1 2.8 2-d S.3 p-3 This figure was…

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Q: 10mL of 1x solution from a 2x stock solution.

A: Dilution formula - C1V1 = C2V2 C1 = concentration of stock C2 = final concentration V1 = volume of…

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Q: 2.) An unknown amino acid was subjected to paper chromatography with five amino acid standards in…

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Q: A chromatography column is used to separate four ami serine, alanine, and histidine. A cylindrical…

A: Chromatography, a group of methods for separating very small quantities of complex mixtures with…

Q: A stock solution was diluted in two steps. In a first step, 0.5 ml was diluted with 9.6 mL buffer.…

A: Formula - Dilution = initial volume / final volume Final concentration = initial concentration ×…

Q: Which of the following indicates buffering in the titration given in the image below? 12 1000000 11…

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Q: 100ml of LB media with 25 μg/ml of Amp and 100 μg/ml of Kan final concentration. You have 100ml of…

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Q: Consider a crude extract with a mixture of the 5 proteins listed below. Protein A 4.5 pl 160…

A: Ion Exchange ChromatographyIon exchange chromatography can be used to separate proteins that have a…

Q: You have a 50X stock solution of TAE buffer, but you need 2 L of a 1X solution for electrophoresis.…

A: TAE buffer used in agarose gel electrophoresis for the seperation of DNA and RNA.

Q: A series of bovine serum albumin (BSA) was prepared and 1 mL of each solution was subjected to a…

A: The Bradford assay is based on the fact that the absorbance maximum for an acidic solution of…

Q: How many cells would you be plating if you pipetted 200uL into your dish from a 125 x 104 cells/mL…

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Q: In UV/Visible spectrophotometer analysis for a multicomponent system, there are only two dyes used…

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Q: I had a protein that was pH 5.6. I then used ion exchnage chromatography with the equilibration…

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Q: Why does it have to be at 470 nm to measure peroxidase activity?

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