2. 1 Calculate the overall charge (pH 7) on the following three polypeptides and answer the questions below. Assume the following pKa values: N-terminal -NH3®, 7.0; all -COOH groups, 4.0%; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. (A) Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys–Pro–Val–Gly-Lys-Lys-Arg-Arg-Pro–Val–Lys–Val-Tyr-Pro-Asp-Ala -Gly- Glu-Asp-Gln– Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu–Phe (B) Ser-Tyr-Ser-Met-Glu–His–Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly–Glu–Glu–Cys–Asp-Pro-Val–Glu–Val–Tyr-Pro-Asp– Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro–Leu-Glu–Phe–Cys–Ser-Tyr-Ser-Met–Glu-His–Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro–Val–Gly-Glu-Glu–Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu-Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala (g) Which of the three peptides would migrate the furthest away from the anode in isoelectric fo- cusing (h) Which of the peptides would migrate the closest to the cathode in isoelectric focusing? (i) Which of the three peptides would migrate the furthest away from the cathode in isoelectric focus- ing? (G) Which of the following three peptides could potentially migrate at a different rate during gel filtra- tion in the absence or in the presence of dithiothreitol (DTT), a compound used for reducing disulfide bridges?
2. 1 Calculate the overall charge (pH 7) on the following three polypeptides and answer the questions below. Assume the following pKa values: N-terminal -NH3®, 7.0; all -COOH groups, 4.0%; Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0. (A) Ser-Tyr-Ser-Met-Glu-His-Phe-Arg-Trp-Gly-Lys–Pro–Val–Gly-Lys-Lys-Arg-Arg-Pro–Val–Lys–Val-Tyr-Pro-Asp-Ala -Gly- Glu-Asp-Gln– Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu–Phe (B) Ser-Tyr-Ser-Met-Glu–His–Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly–Glu–Glu–Cys–Asp-Pro-Val–Glu–Val–Tyr-Pro-Asp– Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro–Leu-Glu–Phe–Cys–Ser-Tyr-Ser-Met–Glu-His–Phe-Asp-Trp-Gly- Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro–Val–Gly-Glu-Glu–Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala (C) Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu-Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala (g) Which of the three peptides would migrate the furthest away from the anode in isoelectric fo- cusing (h) Which of the peptides would migrate the closest to the cathode in isoelectric focusing? (i) Which of the three peptides would migrate the furthest away from the cathode in isoelectric focus- ing? (G) Which of the following three peptides could potentially migrate at a different rate during gel filtra- tion in the absence or in the presence of dithiothreitol (DTT), a compound used for reducing disulfide bridges?
Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
Section: Chapter Questions
Problem 1P
Related questions
Question
Transcribed Image Text:2. .
questions below. Assume the following pKa values: N-terminal –NH3®, 7.0; all -COOH groups, 4.0;
Arg, 12.5; Cys, 8.4; His, 6.0; Lys, 10.0; Tyr, 10.0.
Calculate the overall charge (pH 7) on the following three polypeptides and answer the
(A)
Ser-Tyr-Ser-Met-Glu–His–Phe–Arg-Trp-Gly-Lys-Pro–Val-Gly-Lys-Lys-Arg-Arg-Pro-Val-Lys–Val-Tyr-Pro-Asp-Ala
-Gly- Glu--Asp-Gln- Ser-Ala-Glu-Ala-Phe-Pro-Leu-Arg-Glu-Phe
(B)
Ser-Tyr-Ser-Met-Glu–His–Phe-Arg-Trp-Gly-Ala-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu–Val-Tyr-Pro-Asp-
Ala-Gly-Glu-Asp-Gln-Ser-Ala-Glu-Ala-Phe-Pro-Leu-Glu–Phe-Cys-Ser-Tyr-Ser-Met–Glu–His-Phe-Asp-Trp-Gly-
Asp-Pro-Val-Gly-Pro-Asp-Ala-Gly-Asp-Gln-Pro-Val-Gly-Glu-Glu-Cys-Asp-Pro-Val-Glu-Val-Tyr-Pro-Asp-Ala
(C)
Gly-Ser-Val-Arg-Asp-Pro-Val-Lys-Glu-Val-Tyr-Pro-Asp- Lys-Ala-Gly-Arg-Glu-Ser-Arg-Ala
(g) Which of the three peptides would migrate the furthest away from the anode in isoelectric fo-
cusing
(h) Which of the peptides would migrate the closest to the cathode in isoelectric focusing?
(i) Which of the three peptides would migrate the furthest away from the cathode in isoelectric focus-
ing?
(G) Which of the following three peptides could potentially migrate at a different rate during gel filtra-
tion in the absence or in the presence of dithiothreitol (DTT), a compound used for reducing disulfide
bridges?
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