(2) Determine the sequence of the peptide on the basis of the following data.Amino Acid Composition: (I, K, 2M, P, R, S, T, W, Y)Carboxypeptidase Digestion: (T)Chymotrypsin Digestion: (P, T), (K, M, Y) and (I, M, R, S, W)Cyanogen Bromide Digestion: (M), (K, P, T, Y) and (I, M, R, S, W)Trypsin Digestion: (I, M, R), (P, T, Y) and (K, M, S, W)

Given that the peptide has 10 amino acids. The list of ten amino acids is as follows: Ile (I), Lys…

Answered: (2) Determine the sequence of the…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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5. During prolonged fasting, amino acids derived from muscle proteins and connective tissue become the main substrate for gluconcogenesis. How does glutamate enter the gluconeogenesis? For answer: a) write a reaction of the glutamate conversion into a-ketoglutarate, name the enzyme, indicate the encrgy effect of the reaction; b) draw a diagram for the a-ketoglutarate conversion to oxaloacetate and consequent including of OAA to gluconcogenesis, specify the reactions associated with ATP consumption; c) name the hormones that accelerate gluconeogenesis during fasting and regulatory enzymes of the process; and describe the mechanism of signal transduction by these hormones.
In the intestine, the triacylglycerols must be converted to fatty acids and glycerol by hydrolytic enzymes before transport into enterocytes. Afterward, fatty acids and glycerol are reconverted into triacylglycerols and then packaged into chylomicrons. Suggest why this energy-requiring process is used instead of a direct transport of triacylglycerols into enterocytes.
Adipose triglyceride lipase (ATGL) hydrolyzes a fatty acyl group from: A) monoacylglycerol. B) diacylglycerol. C) triacylglycerol. D) A and C. E) B and C.
Number 7
Which molecular structure (VLDL, LDL, or HDL) is responsible for transporting cholesterol from peripheral tissues to the liver?
The following fragments are produced from a trypsin digest: LPNVSDFTMDCEWQASCR TYILHFN AK LSDEMVCSWQAR The following fragments are produced from a digest with CNBr: VCSWQARLPNVSDFTM AKLSDEM DCEWQASCRTYILHFN What is the original protein sequence?
Consider the decapeptide angiotensin I. Treatment of angiotensin I with ACE (the angiotensin-convertingenzyme) cleaves only the amide bond with the carbonyl group derivedfrom phenylalanine to afford two products. The larger polypeptide isangiotensin II, a hormone that narrows blood vessels and increasesblood pressure. Give the amino acid sequence of angiotensin II usingthree-letter abbreviations. ACE inhibitors are drugs that lower bloodpressure by inhibiting the ACE enzyme
8. The adipose tissue is not only store triacylglycerols but also is active endocrine organ. Explain this function of the adipose tissuc. For that: a) explain the «clinical obesity» and possible consequences of the discase; b) name the biologically active molecules secreted by the adipose tissue; c) explain how secretion of these molecules changes with development of obesity. 9. A daily dict ofa 55-ycar-old woman, consisted ofa 500 g of carbohydrates, 100g of animal fats and 150 g of proteins, at the background of low physical activity.
Using an arrow, draw the site of cleavage for the following peptide that is reacted by: Pepsin (a-b), trypsin (c-d), and chymotrypsin (e) H₂N H SH H H PEPTIDE ACWNEG -NH₂ H H
Answer the following questions based on the structure of the following peptide and the information in the attached table of enzyme activities. M-F-V-W-E-Q-A-P-S-P-L Subjecting this peptide to trypsin would result in OA Met-Phe-Val-Trp-Glu-Gin-Ala-Pro-Ser-Pro-Lys Met-Phe-Val-Trp-Glu-Gin-Ala-Pro-Ser-Pro-Leu OB. Met-Phe-Val-Trp-Glu-Gin-Arg-Pro-Ser-Pro-Leu OC. D. Met-Phe-Val-Trp-Glu-Gin-Arg and Pro-Ser-Pro-Lys O E. Met-Phe-Val-Trp and Glu-Gin-Arg-Pro-Ser-Pro-Leu
Give the 20 amino acids (include structure, name, and symbol) and classify them according to their structures and nutritional requirements.
Number 6

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