Using the standard curve for the digestion of amylose producing maltose (Figure 13, Workshop 4 manual), determine the amount of maltose produced for each of the conditions (Tubes 2-10). Assume that these values are the amount of product (maltose) produced per unit time (e.g., mM per second) or enzyme velocity Absorbance (545 nm) Maltose (mM

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Using the standard curve for the digestion of amylose producing maltose (Figure 13, Workshop 4 manual), determine the amount of maltose produced for each of the conditions (Tubes 2-10). Assume that these values are the amount of product (maltose) produced per unit time (e.g., mM per second) or enzyme velocity

Absorbance (545 nm)

Maltose (mM/sec)

0

 

0

 

0

 

0.040

 

0.210

 

0.330

 

0.295

 

0.280

 

0.202

 

0.030

 

0.6
0.5
0.4
0.3
0.2
0.1
200
400
600
800
1000
Maltose Concentration (mM)
Absorbance (@ 545 nm)
Transcribed Image Text:0.6 0.5 0.4 0.3 0.2 0.1 200 400 600 800 1000 Maltose Concentration (mM) Absorbance (@ 545 nm)
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