You digest the octapeptideMet-Glu-Arg-His-Met-Cys-Phe-Leu with trypsin,chymotrypsin and cyanogen bromide all inthe same beaker. Which of the followingcorrectly lists the isoelectric points for all ofthe fragments that are produced in yourdigest?Relevant pKa values are; 2.2, 4.3, 6.0, 8.3,9.8 and 12.5.5.3, 6.0, 6.0, 7.1, 7.93.3, 6.0, 6.0, 7.1, 7.95.3, 6.0, 6.0, 7.1, 7.9, 11.2O 5.3, 6.0, 6.0, 7.1, 11.25.2, 5.3, 5.3, 7.1, 7.9

An octapeptide has 8 amino acid residues Amino acid sequences of peptides are written with…

Answered: You digest the octapeptide Met-Glu-Arg-…

Biochemistry

9th Edition

ISBN:9781319114671

Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.

Chapter1: Biochemistry: An Evolving Science

Section: Chapter Questions

Problem 1P

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Which of the following condenses acyl and malonyl groups? a) Acetyl co-A ACP transacetylase b) Malonyl co-A ACP transferase c) ß-ketoacyl ACP synthase d) Acyl carrier protein
1. The amino acid sequence for the protein lysozyme is given below. Estimate the isoelectric point for lysozyme protein. The pK, values are provided in Table 3.1. KVFGRCELAAAMKRHGLDNYRGYSLGNWVCAAKFESNFNTQATNRNT DGSTDYGILQINSRWWCNDGRTPGSRNLCNIPCSALLSSDITASVNCAKK IVSDGNGMNAWVAWRNRCKGTDVQAWIRGCRL Here's the sequence in this form: LYS VAL PHE GLY ARG CYS GLU LEU ALA ALA ALA MET LYS ARG HIS GLY Table 3.1 Typical pk, values of ionizable groups in proteins Group Acid Typical pK, Base Terminal a-carboxyl 3.1 group Aspartic acid Glutamic acid 4.1 N. Histidine 6.0 -N + H Terminal a-amino group 8.0 Cysteine 8.3 Тутosine 10.9 + H Lysine 10.8 H H. + N-H Arginine 12.5 N-H N-H Note: Values of pk, depend on temperature, ionic strength, and the microenvironment of the ionizable group. in
g) (CH3)₂ - CH₂ -CH-COOH + CH₂-CH-COOH -H₂O NH₂ OH NH₂ 4. Write the equation for the digestion (hydrolysis) of Phe-Asp-Ala. €
What are the glycosidic linkages of the trisaccharide shown? α1→6, α1→6 α1→6, β1→6 α1→6, β1→5 α1→6, α1→5
Although the first two carbons of fructose and glucose are identical in structure to DHAP and GADP (from glycolysis), DHAP and GADP equilibriate on their in solution to favor the ketone over the aldehyde, while fructose and glucose do not. Why? a)The larger size of the molecule sterically hinders the isomerization b)The larger sugars have more OH groups which hydrogen bond and disrupt isomerization c)The larger sugars cyclize, and there is no carbonyl to isomerize in the cyclic form d)The larger sugars cyclize, and in the cyclic form the hydrogen bonding is very strong e)The larger sugars are less soluble in water than the smaller sugars
Explain the structure and its components
The isoelectric point of an amino acid is the point where; O a) the amino acid carries no net electrical charge b) the a-carboxylic acid is protonated and the a-amino group is unprotonated c) ) the pKa of the a-carboxylic acid is the same as the pK, of the a-amino group a d) the solubility of the amino acid is maximized e) the a-carboxylic acid and the a-amino group are both half protonated MacBo 80 000 esc F1 F2 F3 F4
When the activated form of alcohol is used to synthesize glycerophospholipids, CDP-alcohol reacts with diacylglycerol to form glycerophospholipids. In this metabolic pathway, diacylglycerol is most likely produced by: a) Triacylglycerol lipase b) Phosphatidate phosphorylase c) Diacylglycerol lipase d) All of the above e) None of the above
The α-amylase (α-AM) enzyme secreted by Aspergillus has an isoelectric point of approximately 4.2. If the purification method in problem 1 operates at pH 6.5, would the protein carry a positive charge, negative charge, or net zero charge?
Name the types of glycosidic bonds found in this olygomer, from left to right (from 1 to 3) CH 20H CH 2OH CH 2OH но OH OH OH OH OH OH O alpha1-1, alpha1-4, n O alpha1-4, alpha1-4, n O alpha1-4, beta1-2 O alpha1-4, beta1-4 O beta1-4, alpha 1-4 « Previous Next Not saved Submit Quiz
Determine the sequence of the following 10-mer of ferritin using the data below. i. Complete acid hydrolysis of the 10-mer results in identification of 1A, 1D, 1E, 1F, 1H, 1K, 2L, 1T, 1Y ii. Digestion of the 10-mer with chymotrypsin results in 3 fragments containing (D, H, K, T), (F, L) and (A, E, L, Y) iii. Digestion of the 10-mer with V8 protease, which cuts on the C-side of acidic amino acids, results in three fragments containing (D, F, L, Y), (A, E, L) and (H, K, T) iv. Digestion with trypsin results in a fragment H-T and a fragment with all the other amino acids v. Treatment of the 10-mer with FDNB followed by complete acid hydrolysis results in the following two derivatives, DNP-1 and DNP-2 vi. Treatment of the 10-mer with a carboxypeptidase results in a free threonine
Which of the following is NOT a unit of concentration? All of these options are units of concentration. mM Moles/Liter Molar You gathered your data from Lab , and found the following values: Total Activity of lysozyme in HEW: 14 units Total Activity of lysozyme in carb 1: 21 units Total amount of protein in HEW: 70 mg Total amount of protein in Carb 1: 15 mg Calculate the extent of purification of lysozyme in Carb 1. A 7 fold B 150% C 15 fold D 70%

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