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2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCÀTGggcaacaaa'tgc'tgcagcaagcgacaggcGCCACCATGccGAAtCTGCACAAAccGcATCGCAATCGCAG 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCı _5 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag 1 10 1 Met Pro Asn Leu Gin Gin Thr 10 Ala Ser Gln Ser Gln Linker Kibra

2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCÀTGggcaacaaa'tgc'tgcagcaagcgacaggcGCCACCATGccGAAtCTGCACAAAccGcATCGCAATCGCAG 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCı _5 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag 1 10 1 Met Pro Asn Leu Gin Gin Thr 10 Ala Ser Gln Ser Gln Linker Kibra

Human Anatomy & Physiology (11th Edition)
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCATGggcaacaaatgctgcagcaagcgacaggcGCCACCATGccGAATCTGCAACAAAccGCATCGCAATCGCAGI 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCI 10 10 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag Met Pro Asn Leu Gln Gin Thr Ala Ser Gln Ser Gin Linker Kibra
Transcribed Image Text:2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCATGggcaacaaatgctgcagcaagcgacaggcGCCACCATGccGAATCTGCAACAAAccGCATCGCAATCGCAGI 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCI 10 10 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag Met Pro Asn Leu Gln Gin Thr Ala Ser Gln Ser Gin Linker Kibra
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