2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCÀTGggcaacaaa'tgc'tgcagcaagcgacaggcGCCACCATGccGAAtCTGCACAAAccGcATCGCAATCGCAG 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCı _5 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag 1 10 1 Met Pro Asn Leu Gin Gin Thr 10 Ala Ser Gln Ser Gln Linker Kibra
2) A few years ago, a graduate student in my lab realized that a DNA construct in which he fused the coding sequence for one protein (the 'Myr-tag') to the coding sequence for another protein ('Kibra'), with a short linker between, was not working properly. The goal of this DNA construct was to express a 'fusion protein' which had the Myr-tag fused to the amino terminal end of the Kibra protein. Looking at the portion of the sequence shown below where the two proteins are fused, why did this experiment fail? The brackets above the DNA sequence show the codons corresponding to the amino acids in the proteins shown below the DNA sequence. The translation start site for the fusion protein is indicated. Translation starts,here 5' CACCÀTGggcaacaaa'tgc'tgcagcaagcgacaggcGCCACCATGccGAAtCTGCACAAAccGcATCGCAATCGCAG 3' iGTGGTACccgttgtttacgacgtcgttcgctgtccgCGGTGGTACGGCTTAGACGTTGTTTGGCGTAGCGTTAGCGTCı _5 Met Gly Asn Lys Cys Cys Ser Lys Arg Gln, Myr tag 1 10 1 Met Pro Asn Leu Gin Gin Thr 10 Ala Ser Gln Ser Gln Linker Kibra
Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter19: Genomes And Proteomes
Section: Chapter Questions
Problem 1ITD: Below is a sequence of 540 bases from a genome. What information would you use to find the...
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