12. The following codons and the amino acids they encode is as follows: AUG = Met %3D UUU, UUC = Phe UUA, UUG = Leu UCU, UCC = Ser CCU, CCC = Pro ACU, ACC = Thr %3D %3D %3D %3D The 5' - ACU-UUC-ACU-AUG-UUU-UUA-UCC-UCC-ACU-CCU-UGA-3' MRNA transcript results in a polypeptide. (a) Give the sequence of amino acids in the primary structure of the polypeptide. (3) (b) Give the sequence of nucleotides corresponding to this transcript in: (i) the DNA strand that was read to produce the MRNA (5) (ii) the DNA strand that was NOT read (the coding strand) (5) (c) How many distinct aminoacyl-tRNA synthetases would be used in the synthesis of this polypeptide? (1) (d) Name TWO characteristics of the genetic code that are evident in the data given in the question. (2)

Human Anatomy & Physiology (11th Edition)
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Chapter1: The Human Body: An Orientation
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**Codons and the Amino Acids They Encode:**

- **AUG** = Met
- **UUU, UUC** = Phe
- **UUA, UUG** = Leu
- **UCU, UCC** = Ser
- **CCU, CCC** = Pro
- **ACU, ACC** = Thr

The mRNA transcript: **5' - ACU-UUC-ACU-AUG-UUU-UUA-UCC-UCC-ACU-CCU-UGA - 3'** results in a polypeptide.

**Questions:**

(a) **Amino Acid Sequence**: Identify the sequence of amino acids in the polypeptide's primary structure. (3 marks)

(b) **Nucleotide Sequence**: Provide the nucleotide sequence corresponding to this mRNA transcript in:

   (i) **DNA Template Strand**: The DNA strand that was read to produce the mRNA. (5 marks)

   (ii) **DNA Coding Strand**: The DNA strand that was NOT read (the coding strand). (5 marks)

(c) **Aminoacyl-tRNA Synthetases**: How many distinct aminoacyl-tRNA synthetases are required for this polypeptide's synthesis? (1 mark)

(d) **Genetic Code Characteristics**: Name two characteristics of the genetic code evident from the data given. (2 marks)
Transcribed Image Text:**Codons and the Amino Acids They Encode:** - **AUG** = Met - **UUU, UUC** = Phe - **UUA, UUG** = Leu - **UCU, UCC** = Ser - **CCU, CCC** = Pro - **ACU, ACC** = Thr The mRNA transcript: **5' - ACU-UUC-ACU-AUG-UUU-UUA-UCC-UCC-ACU-CCU-UGA - 3'** results in a polypeptide. **Questions:** (a) **Amino Acid Sequence**: Identify the sequence of amino acids in the polypeptide's primary structure. (3 marks) (b) **Nucleotide Sequence**: Provide the nucleotide sequence corresponding to this mRNA transcript in: (i) **DNA Template Strand**: The DNA strand that was read to produce the mRNA. (5 marks) (ii) **DNA Coding Strand**: The DNA strand that was NOT read (the coding strand). (5 marks) (c) **Aminoacyl-tRNA Synthetases**: How many distinct aminoacyl-tRNA synthetases are required for this polypeptide's synthesis? (1 mark) (d) **Genetic Code Characteristics**: Name two characteristics of the genetic code evident from the data given. (2 marks)
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