10. Here is a graph describing the kinetics of PCR. Answer the questions below. PCR Product (Log 2) C B A PCR Cycle Number i. Reaction is in what phase when it is at point A in the graph? ii. Reaction is in what phase when it is at point B in the graph? iii. Reaction is in what phase when it is at point C in the graph? Name of Phase: 11. ✓ ✓ ✓ DNA DNA was isolated, diluted 1:100 in water, and an optical density (OD) of 0.12 was measured at 260nm." What is the concentration of the DNA solution if you assume that an optical density of 1.0 OD 260 is (2. equivalent to 50ng/μl? 12. (stock solution has a concentration of 3.5 X 10° target DNA molecules/al.. a. If you make a 1:100 dilution of this stock, what is the resulting concentration of the DNA? b. If you add 5 μl of the diluted DNA in part a (above) to a PCR reaction tube. How many target DNA molecules did start with in the PCR reaction tube? 4

10. Here is a graph describing the kinetics of PCR. Answer the questions below. PCR Product (Log 2) C B A PCR Cycle Number i. Reaction is in what phase when it is at point A in the graph? ii. Reaction is in what phase when it is at point B in the graph? iii. Reaction is in what phase when it is at point C in the graph? Name of Phase: 11. ✓ ✓ ✓ DNA DNA was isolated, diluted 1:100 in water, and an optical density (OD) of 0.12 was measured at 260nm." What is the concentration of the DNA solution if you assume that an optical density of 1.0 OD 260 is (2. equivalent to 50ng/μl? 12. (stock solution has a concentration of 3.5 X 10° target DNA molecules/al.. a. If you make a 1:100 dilution of this stock, what is the resulting concentration of the DNA? b. If you add 5 μl of the diluted DNA in part a (above) to a PCR reaction tube. How many target DNA molecules did start with in the PCR reaction tube? 4

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 16SQ: Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA...

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= Menu #1 Mol Bio Restriction An... X + Create All tools Edit Convert E-Sign Sign in Find text or tools H 3. If the PMBBS plasmid were digested with all three REs together, how many fragments would be observed, and what would be the sizes (in bp) of these fragments? Number of fragments: Sizes: 4. Draw the actual map of the PMBBS plasmid, following the style of the sample map shown in Figure 1. Make sure you show the following information: (1) relative locations of the RE sites; (2) size in base pairs of the segments of DNA between sites. (The map need not be drawn to scale.) Answer: (provide a drawing) NFL Q Search IA W ☑ 0 Al Assistant C 2 2 C 1:1 ༢ ☑ 8:58 PM 10/22/2024 Q
(a) To illustrate how the ADP phosphorylative capacity was measured, the diagram below on the left shows a ³1P NMR spectrum of the arm of a control female subject at rest, and the diagram on the right the changes measured in the creatine and phosphocreatine levels of the arm at rest and exercise. PCr 10 Exer -cise Exer →→→Rest◄cise Rest PCr/Pi 9 PCr 15 20 10 Time(min) Isokinetic ergometer exercise of the control female subject showing intervals of rest and exercise. To obtain this graph, a ³1P NMR spectrum was col- lected every 2 min to show the changes in the PCr and Pi content of the arm skeletal muscle tissue. NH₂ during activity + ATP + C=NH2 during recovery YATP Pi GATP BATP SP 15 0 10 15 20 25 5 ppm 31P NMR spectrum of the arm of a control sub- ject at rest showing characteristic resonance features for the phosphate residues of ATP, phosphocreatine (PCr), and Pi. At rest the PCr/Pi ratio is ordinarily ~ 8-9. The synthesis of phosphocreatine is unfavorable. If dur- ing extended…
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You buy a new PCR kit.  The starting concentration for each reagent is as follows: the primers are 200 mM (mmole/L), the PCR buffer + dNTPs is 150 mM (mmole/L), and the polymerase 15 units/mL. In the protocol manual, it states that you want the final concentration (FC) of primers to be 40 mM each, the FC of PCR buffer + dNTPs to be 25 mM, and to have 4 units of the DNA polymerase in the final 50 mL PCR reaction.
1. The final amount of each primer required in a PCR reaction is 0.025 nanomol. If the total volume of the PCR reaction is equal to 100 µl and the stock concentration of each primer is equal to 10 µM. Calculate the volume of stock primer that needs to be added in order to ensure a primer amount of 0.025 nanomol. 2. A 250 ml phosphate buffer solution contains 204.12 µg KH2PO4 and 87.09 µg K2HPO4, respectively. Determine the molarities in micromolar (µM) of the abovementioned compounds in the phosphate buffer solution. [Mw KH2PO4 = 136.08 g/mol and Mw K2HPO4 = 174.18 g/mol] 3. You are required to use the pET 28b expression vector in a cloning experiment. If the vector is 5.368 kilobasepairs in length and the mass of the DNA that you have added represent 8.5 x 109 molecules. Determine the mass of vector DNA that you have added to your reaction? [1basepair ~ 660 Da or g/mol, Avogadro’s constant = 6.023 x 1023 molecules/mol]
Answer the table below:
A target gene for producing human insulin must be inserted into a plasmid before the transformation process. Why the plasmid is important in this process? * I The target gene lacks the ability to replicate itself. II Screening process will be difficult without cloning vector. II The host cell is able to accept the recombinant plasmid but not the non- recombinant plasmid. O I and Il only I and III only O Il and III only O I, Il and III
at Arrange Window Help 4) 57% D Tue 4 May Tools Slide Show 2 ARMS PCR nations Slide Show Review View A A =、=<|EE|三v| v v A Sha v 20 Quick Styles D Sha Text Arrange Convert to SmartArt Picture Shapes ab x x. AV v Aa v D. Av A、申。 Box Briefly outline how MLPA probe-sets can simultaneously detect multiple targets using a single set of PCR primers. E Notes Comments MAY 4 Pr w MacBook Air
2 of 11 Identify the incorrect statements O Taq polymerase can incorporate nucleotides at a rate of -60bp per second at 70°C It is possible to re-amplify PCR products to obtain a higher yield of the target Steps in PCR are typically repeated (cycled) 25-35 times The maximum PCR product length that Taq polymerase can typically amplify is approximately 10 kbp V Extension is the second step in PCR
Slide 8 Please help me explain this on my report on cell dogma
Why is the company Qiagen has more refined DNA extraction steps than a normal Strawberry DNA extraction practical? Summary of Qiagen DNA extraction steps Add ATL buffer and grind with sample. Add 20 microliters of enzyme Proteinase K to degrade protein into a 1.5-2ml microcentrifuge tube. Add 200 microlitres AL lysis buffer, and mix by vortexing for 5–10 seconds, which breaks cell membrane allowing DNA to be released. Incubate the sample at 56 degrees for 10 minutes. Mix the cell lysate with 200 microlitres ethanol by pipetting it at the side of the microcentrifuge wall so DNA precipitates. The DNA forms a white layer and the remaining liquid is discarded. Pipet the mixture into DNeasy Mini spin column placed in a 2 ml collection tube. Centrifuge for a minute at 8000 rpm. Place the mini spin column into a 2 ml collection tube, add 500 µl Buffer AW1, and centrifuge for 1 min at 8000 rpm. Then add it to a new 2 ml collection tube (provided), add 500 µl Buffer AW1, and centrifuge for 1…
177 3 The figure above represents results observed from the differential gene expression lab. Lane 3 corresponds to a PCR positive result and Lane 4 represents a PCR negative. Which of the following would represent E. coli grown in 0.5% glucose only media? O Lane 1 Lane 2