10. Here is a graph describing the kinetics of PCR. Answer the questions below. PCR Product (Log 2) C B A PCR Cycle Number i. Reaction is in what phase when it is at point A in the graph? ii. Reaction is in what phase when it is at point B in the graph? iii. Reaction is in what phase when it is at point C in the graph? Name of Phase: 11. ✓ ✓ ✓ DNA DNA was isolated, diluted 1:100 in water, and an optical density (OD) of 0.12 was measured at 260nm." What is the concentration of the DNA solution if you assume that an optical density of 1.0 OD 260 is (2. equivalent to 50ng/μl? 12. (stock solution has a concentration of 3.5 X 10° target DNA molecules/al.. a. If you make a 1:100 dilution of this stock, what is the resulting concentration of the DNA? b. If you add 5 μl of the diluted DNA in part a (above) to a PCR reaction tube. How many target DNA molecules did start with in the PCR reaction tube? 4

10. Here is a graph describing the kinetics of PCR. Answer the questions below. PCR Product (Log 2) C B A PCR Cycle Number i. Reaction is in what phase when it is at point A in the graph? ii. Reaction is in what phase when it is at point B in the graph? iii. Reaction is in what phase when it is at point C in the graph? Name of Phase: 11. ✓ ✓ ✓ DNA DNA was isolated, diluted 1:100 in water, and an optical density (OD) of 0.12 was measured at 260nm." What is the concentration of the DNA solution if you assume that an optical density of 1.0 OD 260 is (2. equivalent to 50ng/μl? 12. (stock solution has a concentration of 3.5 X 10° target DNA molecules/al.. a. If you make a 1:100 dilution of this stock, what is the resulting concentration of the DNA? b. If you add 5 μl of the diluted DNA in part a (above) to a PCR reaction tube. How many target DNA molecules did start with in the PCR reaction tube? 4

Biology: The Unity and Diversity of Life (MindTap Course List)

15th Edition

ISBN:9781337408332

Author:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Publisher:Cecie Starr, Ralph Taggart, Christine Evers, Lisa Starr

Chapter15: Studying And Manipulating Genomes

Section: Chapter Questions

Problem 16SQ: Match the method with the appropriate enzyme. _____ PCR a. Taq polymerase _____ cutting DNA b. DNA...

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n gel electrophoresis of DNA, the different bands in the final gel form because the DNA molecules _______. a. are from different organisms b. have different lengths c. have different nucleotide compositions d. have different genes
After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
PCR can be used ______. a. as a cloning vector b. in DNA profiling c. to modify a human genome
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
When Griffith injected mice with a combination of live rough-strain and heat-killed smooth-strain pneumococci, he discovered that (a) the mice were unharmed (b) the dead mice contained living rough-strain bacteria (c) the dead mice contained living smooth-strain bacteria (d) DNA had been transferred from the smooth-strain bacteria to the mice (e) DNA had been transferred from the rough-strain bacteria to the smooth-strain bacteria
Which of the following is not correct for bacterial conjugation? a. Both Hfr and F+ bacteria have the ability to code for a sex pilus. b. After an F- cell has conjugated with an F+, its plasmid holds the F+ factor. c. The recipient cell is Hfr following conjugation. d. In an HfrF- conjugation, DNA of the main chromosomemoves to a recipient cell. e. Genes on the F factor encode proteins of the sex pilus.
Match each term with the most suitable description. ______ DNA profile a. GMO with a foreign gene ______ Ti plasmid b. alleles commonly have them ______ probes c. unique array of short tandem repeats ______ SNPs d. used to find clones. ______ transgenic e. mouse vs. human ______ genomics f. used in plant gene transfers ______ CRISPR g. based on RNA interference
During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase
Meselson and Stahl's experiments proved that DNA replicates by which mode? conservative semi-conservative dispersive none of the above
Information obtained by microscopic analysis of stained chromosomes is used in: radiation hybrid mapping sequence mapping RFLP mapping cytogenetic mapping
DNA molecular with complementary sticky ends associate by (a) covalent bonds (b) hydrogen bonds (c) ionic bonds (d) disulfide bonds (e) phosphodiester linkages
Which of the following best describes the process of DNA sequencing? a. DNA is separated on a gel, and the different bands are labeled with fluorescent nucleotides and scanned with a laser. b. A laser is used to fluorescently label the nucleotides present within the DNA, the DNA is run on a gel, and then the DNA is broken into fragments. c. Nucleotides are scanned with a laser and incorporated into the DNA that has been separated on a gel, and then the DNA is amplified with PCR. d. Fragments of DNA are produced in a reaction that labels them with any of four different fluorescent dyes, and the fragments then are run on a gel and scanned with a laser. e. DNA is broken down into its constituent nucleotides, and the nucleotides are then run on a gel and purified with a laser.