1. To do this question, DNA sequence coding the Homo sapiens phenylalanine hydroxylase (PAH) gene below is referred. Nucleotide Sequence (1359 nt): ATGTCCACTGCGGTCCTGGAAAACCCAGGCTTGGGCAGGAAACTCTCTGACTTTGGACAGGAAACAAGCT| ATATTGAAGACAACTGCAATCAAAATGGIGCCATATCACTGATCTTCTCACTCAAAGAAGAAGTTGGTGC ATTGGCCAAAGTATTGCGCTTATTTGAGGAGAATGATGTAAACCTGACCCACATTGAATCTAGACcCTTCT CGTTTAAAGAAAGATGAGTATGAATTTTTCACCCATTIGGATAAACGTAGCCTGCCTGCTCTGACAAACA ТСАТCAAGATCтIGAGGCATGAСATTGGTGCCACтстсCATGAGCTттІCACGAGATAAGAAGAAAGACAС AGTGCCCTGGTТСССАAGAACCАTTCAAGAGCTGGACAGATTIGCСААТСAGATTCTCAGCTATGGAGCG GAACTGGATGCTGACCACCCTGGTTTTAAAGATCCTGTGTACCGTGCAAGACcGGAAGCAGTTTGCTGACA тIссCТАCAAСТАСССССАТGGСCAGCCCATCССтCGAGTGGAATАСATGGAGGAAGAAAAGAAAACAтG GGGCACAGTGTTCAAGACTCTGAAGTCCTTGTATAAAACCCATGCTTGCTATGAGTACAATCACATTTTT CCACTTCTTGAAAAGTACTGTGGCTTCCATGAAGATAACATTCCCCAGCTGGAAGACGTTTCTCAGTTCC TGCAGACTTGCACTGGTTTCCGCCTCCGACCTGTGGCTGGCCTGCTTTCCTCTCGGGATTTCTTGGGTGG сстGсссттссGAGTCTTCCACTGCACАCAGTACATCAGACATGGATCCAAGCCCATGTATACCCCCGAА CCTGACATCTGCCATGAGCTGTTGGGACATGTGCCCTTGTTTTCAGATCGCAGCTTTGCCCAGTTTTCCC AGGAAATTGGCCTTGCCTCTCTGGGTGCACCTGATGAATACATTGAAAAGCTCGCCACAATTTACTGGTT TACTGTGGAGTTTGGGCTCTGCAAACAAGGAGACTCCATAAAGGCATATGGTGCTGGGCTCCTGTCATCC TTTGGTGAATTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCA TCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGA GAAAGTAAGGAAСTTTCCTGCCACAАТАССТСGGCCCттстCAGTTCGCTACGACCCATACACCCАAAGG ATTGAGGTCTTGGAСAАTАCССAGCAGCТТAAGATTTIGGCTGATTCCATTAACAGTGAAATTGGAATCC TTTGCAGIGCCCTCCAGAAAATAAAGTAA Perform a web search for the restriction site that is recognized by the restriction enzyme NcoI. Next, figure out how many NcoI sites are in the PAH sequence. Highlight the restriction sites.

Biochemistry
6th Edition
ISBN:9781305577206
Author:Reginald H. Garrett, Charles M. Grisham
Publisher:Reginald H. Garrett, Charles M. Grisham
Chapter28: Dna Metabolism: Replication, Recombination, And Repair
Section: Chapter Questions
Problem 16P: Helicase Unwinding of the E. coli Chromosome Hexameric helicases, such as DnaB, the MCM proteins,...
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1. To do this question, DNA sequence coding the Homo sapiens phenylalanine hydroxylase (PAH) gene
below is referred.
Nucleotide Sequence (1359 nt):
АTСтCCАСТGCGGTCCTGGAAААСССAGGCTTGGGCAGGAAAстстстGACTTTGGACAGGAAAСAAGCT
АТАТTGAAGАСAАСТGCAAТСАAAАТGGTGCCATATCАСTGATCTTCTCАСТСААAGAAGAAGTTGGTGС
ATTGGCCAAAGTATTGCGCTTATTTGAGGAGAATGATGTAAACCTGACCCACATTGAATCTAGACCTTCT
CGTTTAAAGAAAGATGAGTATGAATTTTTCACCCATTTGGATAAACGTAGCCTGCCTGCTCTGACAAACA
TCATCAAGATCTTGAGGCATGACATTGGTGCCACTGTCCATGAGCTTTCACGAGATAAGAAGAAAGACAC
AGTGCCCTGGTTCCCAAGAACCATTCAAGAGCTGGACAGATTTGCCAATCAGATTCTCAGCTATGGAGCG
GAACTGGATGCTGACCACCCTGGTTTTAAAGATCCTGTGTACCGTGCAAGACGGAAGCAGTTTGCTGACA
ттссCТАCAАСТАСССССАТGGCCAGCCCATCсстсGAGTGGААТАСАТGGAGGAAGAAAAGAAААСАТG
GGGCACAGTGTTCAAGACTCTGAAGTCCTTGTАТАAААСССАтсCTTGCTATGAGTACAATCACАTTTT
ССАСТтсттGАAAAGTАСТGTGGCTTCCATGAAGATAACATTCсссСAGCTGGAAGACGTTTCTCAGTTCC
TGCAGACTTGCACTGGTTTCCGCCTCCGACCTGTGGCTGGCCTGCTTTCCTCTCGGGATTTCTTGGGTGG
CCTGGCCTTCCGAGTCTTCCACTGCACACAGTACATCAGACATGGATCCAAGCCCATGTATACCCCCGAA
CCTGACATCTGCCATGAGCTGTTGGGACATGTGCCCTTGTTTTCAGATCGCAGCTTTGCCCAGTTTICCC
AGGAAATTGGCCTTGCCTCTCTGGGTGCACCTGATGAATACATTGAAAAGCTCGCCACAATTTACTGGTT
TACTGTGGAGTTTGGGCTCTGCAAACAAGGAGACTCCATAAAGGCATATGGTGCTGGGCTCCTGTCATCC
TTTGGTGAATTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCA
TCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGA
GAAAGTAAGGAACTTTGCTGCCACAATАCСТCGGCCCTTСТСAGTTCGCTАCGACCCATAСАСССАААGG
ATTGAGGTCTTGGACAATACCCAGCAGCTTAAGATTTTGGCTGATTCCATTAACAGTGAAATTGGAATCC
TTTGCAGTGCCCTCCAGAAAATAAAGTAA
Perform a web search for the restriction site that is recognized by the restriction enzyme NcoI. Next,
figure out how many NcoI sites are in the PAH sequence. Highlight the restriction sites.
Transcribed Image Text:1. To do this question, DNA sequence coding the Homo sapiens phenylalanine hydroxylase (PAH) gene below is referred. Nucleotide Sequence (1359 nt): АTСтCCАСТGCGGTCCTGGAAААСССAGGCTTGGGCAGGAAAстстстGACTTTGGACAGGAAAСAAGCT АТАТTGAAGАСAАСТGCAAТСАAAАТGGTGCCATATCАСTGATCTTCTCАСТСААAGAAGAAGTTGGTGС ATTGGCCAAAGTATTGCGCTTATTTGAGGAGAATGATGTAAACCTGACCCACATTGAATCTAGACCTTCT CGTTTAAAGAAAGATGAGTATGAATTTTTCACCCATTTGGATAAACGTAGCCTGCCTGCTCTGACAAACA TCATCAAGATCTTGAGGCATGACATTGGTGCCACTGTCCATGAGCTTTCACGAGATAAGAAGAAAGACAC AGTGCCCTGGTTCCCAAGAACCATTCAAGAGCTGGACAGATTTGCCAATCAGATTCTCAGCTATGGAGCG GAACTGGATGCTGACCACCCTGGTTTTAAAGATCCTGTGTACCGTGCAAGACGGAAGCAGTTTGCTGACA ттссCТАCAАСТАСССССАТGGCCAGCCCATCсстсGAGTGGААТАСАТGGAGGAAGAAAAGAAААСАТG GGGCACAGTGTTCAAGACTCTGAAGTCCTTGTАТАAААСССАтсCTTGCTATGAGTACAATCACАTTTT ССАСТтсттGАAAAGTАСТGTGGCTTCCATGAAGATAACATTCсссСAGCTGGAAGACGTTTCTCAGTTCC TGCAGACTTGCACTGGTTTCCGCCTCCGACCTGTGGCTGGCCTGCTTTCCTCTCGGGATTTCTTGGGTGG CCTGGCCTTCCGAGTCTTCCACTGCACACAGTACATCAGACATGGATCCAAGCCCATGTATACCCCCGAA CCTGACATCTGCCATGAGCTGTTGGGACATGTGCCCTTGTTTTCAGATCGCAGCTTTGCCCAGTTTICCC AGGAAATTGGCCTTGCCTCTCTGGGTGCACCTGATGAATACATTGAAAAGCTCGCCACAATTTACTGGTT TACTGTGGAGTTTGGGCTCTGCAAACAAGGAGACTCCATAAAGGCATATGGTGCTGGGCTCCTGTCATCC TTTGGTGAATTACAGTACTGCTTATCAGAGAAGCCAAAGCTTCTCCCCCTGGAGCTGGAGAAGACAGCCA TCCAAAATTACACTGTCACGGAGTTCCAGCCCCTCTATTACGTGGCAGAGAGTTTTAATGATGCCAAGGA GAAAGTAAGGAACTTTGCTGCCACAATАCСТCGGCCCTTСТСAGTTCGCTАCGACCCATAСАСССАААGG ATTGAGGTCTTGGACAATACCCAGCAGCTTAAGATTTTGGCTGATTCCATTAACAGTGAAATTGGAATCC TTTGCAGTGCCCTCCAGAAAATAAAGTAA Perform a web search for the restriction site that is recognized by the restriction enzyme NcoI. Next, figure out how many NcoI sites are in the PAH sequence. Highlight the restriction sites.
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