1. Describe the role of Cys in the catalytic mechanism shown. 2. Describe/predict the microenvironment that could allow for Cys to be ionized in the catalytic mechanism.
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- Using the ActiveModel for phosphofructokinase (Trypanosoma), describe the difference between the APO1, AP02, and holoenzyme conformations.S 1 CH₂ ✪ NH3 0=0 H₂C HN 6 NH CH₂ 'S Co A L CH₂ NH 4. Describe the role of His in the catalytic mechanism shown.Catalane in un enzyme that speeds up the decomposition ot hydrogen peroxide H0) to waler and axvgen. Students conducted two investigationa to determine the ideal conditions for the function of catalane. One wetigation compared catalase activity at diferent values of pH. The other imvestigation compared catalane activity al diferent temperatures. Enayme Activity ve pH Enzyme Aetivity vs Temperature Teate C) According to the data in the graphs, which pH and temperature combination provides the BEST conditions for catalase to tunction? Anury lag a
- SPS Date: Page No. A pulse af 13-14c] of pyruate con tans 4c) js methylgiaup. added toa do0lated mitachondria. Prruiate (the 'Riuspension of solated mitochondoria Aften (Q ane tuen of c gcid cycle, which canlion co e (ore) labeled in Daaloocetate? he citri'c loore) labeledin Draw f caid crle infermedictes t shau hene the labreled the structures ALL the cetrec tohone canban ie ih erch one. (t How needed to nelecse caribon many tong the Gycle would te Eycle,Wou all the labeled as 1"cog? Explain your answer.During Cytochrome P450-catalyzed monooxygenation... ... iron is being both reduced and oxidized, while other atoms never change their oxidation state iron is being reduced, while hydrogen is being oxidized O O O ... oxygen is being reduced, while hydrogen is being oxidized carbon is being reduced, while hydrogen is being oxidized carbon is being oxidized, while oxygen is being reducedAct on substroate Assignmest Talce a partculor medicinal plant, daw out the metabolic pathway leg Vemenia amygdahing)imlues cn that plest metabolite popular in that medicinal plant and enzyme it's formetion to get a petiuler seerdary responsible for
- 2 Construct a PyMOL image to show the overall fold (including the secondary structure elements), quaternary structure, CPD an inhibitor and NADPH bound, and Mg²+ binding in the S. aureus KARI (PDB: 5W3K). Attach a figure legend ~100-200 words.AA View Tell me Convert to SmartArt W- Picture Shapes - Text Box Arrange Quick d Tue Dec 6 3:13 PM ♫ Share Design Qsn 2 (a) A pathway consists of 6 enzymes (p, q, r, s, t, u) that convert substrate J to product X at a rate of 10 moles/hour. If enzyme q is increased from 5 mmoles to 35 mmoles the amount of product X increases to 20.5 moles/hour. What is the flux control coefficient for enzyme q (C¹)?This is a linear biochemical pathway 2 ATP + HCO₂ + NH₂ 2 ADP, PCPS-1 H₂N OPO, NH₂ CH₂ CH₂ CH₂ HỘ NHĨ coo 1 H₂N NH₂ 7 True False ARG1 P отс fessels. çoo CH HC 5 Sassets. ASL NH₂ HỘ NHỎ ASS N_y_coo NH CHI CH₂COO CH₂ CH₂ HỘ NHỎ coo 4 ATP PP L-Asp AMP 1 L-ornithine 2 carbamoyl phosphate 3 L-citrulline 4 argininosuccinate 5 fumarate 6 L-arginine 7 urea L-Asp L-aspartate CPS-1 carbamoyl phosphate synthetase I OTC Ornithine transcarbamoylase ASS argininosuccinate synthetase ASL argininosuccinate lyase ARG1 arginase 1 OH OH NH₂
- 21. The figure below illustrates two )Giving r expected A al im flasks were pathways of respiratory reactions in man. ment instead of d)why wer instead n flask B were: lin. For the results re set-up after Pathway A: Glucose 1. Define (i) Exe (ii) Ho: (iii) Ege (iv) Sec H,O+CO,+ Energy Pathway B: Glucose Lactic acid + Energy were used to east in glucose n(IV) oxide in (a) Identify the type of respiration represented by (i) Pathway A (ii) Pathway B (b) State two disadvantages of pathway B. 2. List do substar 3. Name t substar 22. The following experiment was set up to investigate whether energy is released by yeast cells. Solution in flask A andB were kept at 30°C during the experiment. 4. Name t amoeba 5. Comple Thermometer remove the 10% Organ i) Sk ii) iii) Lu Vacuum flasks = the ... 3. pen to the riment Oil layer Distilled water 6. Na ppen if the solution Water + 10% Glucose solution + yeast Flask A Flask B 7. (i) room a)Give the purpode of the oil layer 'above b)State the purpose of…NH3 H-SCO A LES NH CH₂ NH CH₂ CH₂ 3 H-SCO A H3C SCO A 5. Describe the role of His in the catalytic mechanism shown.Understanding the Mechanisms of Reactions Related to Transketolase The mechanistic chemistry of the acetolactate synthase and phosphoketolase reactions (shown here) is similar to that of the transketolase reaction (Figure 22.30). Write suitable mechanisms for these reactions.
