18:20 O O OO0 •4G+* ll 7%Restriction enzyme tutorial 2021_1_ - Saved1. Calculate the size of the resulting fragments as they will occur after digestion and writethe sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (seefigure 3A).%3DBBamHI530522,34627,9723449941,732op)ECORI,21,226 26,104 31,74739,16844,972(bp)DHindll23,130 27,479 36,895 37,584 4414125,157(bp)37,4592. How many fragments would you expect to see for each of the maps indicated inquestion 1 above?Мар В:Мар С:Мар D:3. Use the sequence indicated in figure 1 (also refer to figure 2 and 3) and indicate howmany times does the sequence GAATTC occur in the A DNA sequence? What aboutAAGCTT and GGATCC?GGATC:GAATTC:АAGCTT:B IU e A = E 18:20 O O OO0 •4G+* ll 7%Restriction enzyme tutorial 2021_1_ - Saved4. Draw these fragments as they would migrate on the electrophoresis gel. Write the sizeof the fragments on the square when you draw them.5. According to your answers provided at question 2 there should be a certain amount ofLadderBamHIEcoRIHindllbp50,00024,00020,00016,00010,0009,0008,0007,0006,0005,0004,0003,0002,0001,000bands visible on your gel. Suppose you did this practical and you observed less bandson your gel, give possible explanations for this.B IU eDNA migration||

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18:20 O O OO0 •
4G+
* ll 7%
Restriction enzyme tutorial 2021_1_ - Saved
1. Calculate the size of the resulting fragments as they will occur after digestion and write
the sizes on the maps below. Note that linear DNA has a total size of 48 502 bp (see
figure 3A).
%3D
B
BamHI
5305
22,346
27,972
34499
41,732
op)
ECORI,
21,226 26,104 31,747
39,168
44,972
(bp)
D
Hindll
23,130 27,479 36,895 37,584 44141
25,157
(bp)
37,459
2. How many fragments would you expect to see for each of the maps indicated in
question 1 above?
Мар В:
Мар С:
Мар D:
3. Use the sequence indicated in figure 1 (also refer to figure 2 and 3) and indicate how
many times does the sequence GAATTC occur in the A DNA sequence? What about
AAGCTT and GGATCC?
GGATC:
GAATTC:
АAGCTT:
B IU e A = E

18:20 O O OO0 •
4G+
* ll 7%
Restriction enzyme tutorial 2021_1_ - Saved
4. Draw these fragments as they would migrate on the electrophoresis gel. Write the size
of the fragments on the square when you draw them.
5. According to your answers provided at question 2 there should be a certain amount of
Ladder
BamHI
EcoRI
Hindll
bp
50,000
24,000
20,000
16,000
10,000
9,000
8,000
7,000
6,000
5,000
4,000
3,000
2,000
1,000
bands visible on your gel. Suppose you did this practical and you observed less bands
on your gel, give possible explanations for this.
B IU e
DNA migration
||

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Can you please answer question 5.

According to your answers provide at question 2 there should be a certain number of bands visible on your gel. Suppo. you did this practical and you observed less bands on your gel, give possit I explanations for this.

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